Submitted to: Mid Atlantic Plant Molecular Biology Society Conference
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2005
Publication Date: 4/1/2005
Citation: Cooper, B., Garrett, W.M., Campbell, K. 2005. Shotgun identification of proteins from rust spores. Mid Atlantic Plant Molecular Biology Society Conference .
Technical Abstract: We are interested in learning more about the proteome of Uromyces appendiculatus, the causal agent of common bean rust. The knowledge of the proteins that form uredospores, germlings, appressoria, and haustoria may be used to define host-pathogen interactions or serve as targets for chemical inhibition of the fungus. We have used liquid chromatography tandem mass spectrometry (LC-MS/MS) in a high-throughput fashion to identify ~400 proteins from uredospores. An abundance of heat-shock proteins and translation elongation factors portends a spore’s ability to survive stresses and jump-start protein production when germination is initiated.