Submitted to: Genbank
Publication Type: Abstract Only
Publication Acceptance Date: 6/2/2005
Publication Date: 6/27/2005
Citation: Proszkowiec-Weglarz, M., Richards, M.P., Poch, S.M. 2005. Gallus gallus 5'-AMP-activated protein kinase gamma-3 non-catalytic subunit long form (PRKAG3) mRNA, complete cds. GenBank Accession Number DQ079814.
Technical Abstract: AMP-activated protein kinase (AMPK) is a sensor of cellular energy charge. Once activated by an increase in the AMP:ATP ratio, AMPK acts as a master switch to increase energy-generating and to reduce energy-consuming metabolic pathways. AMPK exists as an enzyme complex consisting of one catalytic subunit (alpha) and two regulatory subunits (beta and gamma). A molecular cloning strategy involving primer-directed reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of 5’ cDNA ends (5’ RACE) was developed to sequence 1788 base pairs (bp) of a chicken breast muscle–derived cDNA corresponding to the complete coding region and portions of the 5’- and 3’-untranslated regions of the chicken AMPK gamma-3 long form mRNA transcript variant. Alternative splicing of exon 3 in the AMPK gamma-3 gene resulted in the inclusion of an additional 11 bp of sequence and a shift in the reading frame of the long form mRNA transcript. The chicken AMPK gamma-3 long form mRNA codes for a predicted 382 amino acid protein which contains four cystathionine beta-synthase (CBS) domains. This sequence data confirmed for the first time the existence and expression of the AMPK gamma-3 long form mRNA in chickens. Such data will be useful in studying the structure and expression of the chicken AMPK gamma-3 gene, as well as, in further defining the function of the AMPK pathway in poultry.