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Title: ROOM TEMPERATURE STORAGE EFFECTS ON PICM-19H CELL FUNCTION: ARTIFICIAL LIVER DEVICE IMPLICATIONS

Author
item Mahmood, Ayesha
item Hwang, David
item Talbot, Neil
item Caperna, Thomas

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/28/2005
Publication Date: 9/28/2005
Citation: Mahmood, A., Hwang, D., Talbot, N.C., Caperna, T.J. 2005. Room temperature storage effects on PICM-19H cell function: artificial liver device implications. Proceedings of the Biomedical Engineering Society.

Interpretive Summary:

Technical Abstract: Extracorporeal artificial liver device (ALD) support systems may offer a feasible approach for patients suffering from liver disease. The overall objective of this work is to develop an ALD using the pig epiblast-derived PICM-19H cell line. PICM-19H cells exhibit hepatocyte functions including ammonia clearance capacity and P450 activity. A unique characteristic of PICM-19H cells is their ability to remain viable and functional after room temperature (RT) storage. Here, we report RT storage effects on PICM-19H hepatocyte function. PICM-19H cells were co-cultured with STO feeder cells for 4 weeks in T-25 flasks. The resulting differentiated and confluent cultures were exposed to RT (24oC) for 10, 6, 3, 1, 0 days. Thereafter, all RT treated flasks were incubated at 37oC for 1 day recovery. Cell viability (WST-1 metabolism), ammonia clearance (2mM), urea production and EROD activity were then measured. The results indicated that PICM-19H cells retain their ability to convert ammonia into urea and also maintain EROD activity after being exposed to RT for up to 6 days. WST-1 uptake metabolic rates somewhat decreased by 10 day RT storage. However, the cellular metabolic activity per unit protein was comparable regardless of RT exposure time. Current work is focused on evaluating cellular activity as a function of temperature, incubation time, and post-incubation recovery time. Results from this study will be useful in product assembly, packaging and shelf-life of the device.