Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2005
Publication Date: 6/19/2005
Citation: Rieder, A.E. 2005. Development of a Mammalian Cell-free System to Investigate Replication of Foot-and-Mouth Disease and the Role of Host Factors in Virus Synthesis. XIIIth Meeting of the European Study Group for the Molecular Biology of Picornaviruses. P.H24
Technical Abstract: Foot-and-mouth disease (FMD) has been recognized as one of the most contagious viral disease of cloven-hoofed animals with important socioeconomic implications for animal product market and trade. Although the causative agent FMDV was first described in the 16th century, there are significant gaps in our understanding of the molecular aspects of FMDV pathogenesis. Most notably is the lack of information regarding host factors involved in viral RNA replication, as well as the mechanism of virus host range and virulence. We have established a coupled translation-RNA replication system that combines extracts from uninfected BHK-21 cells and the viral RNA to achieve de novo virus synthesis in the test tube. At optimal Mg2+ and RNA concentrations virus titers in the order of 10 to the 5th power pfu/ml were obtained for various FMDV serotypes including type O,A and SAT, and the virus progeny displayed similar phenotype as the parental strains. Currently we are using this cell-free system, which is programmed with FMDV RNA, to analyze the kinetics of virus protein and RNA synthesis and to evaluate the mode of action of antiviral compounds acting on early stages of virus synthesis.