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item Cooper, Bret

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 7/31/2005
Publication Date: 7/31/2005
Citation: Cooper, B. 2005. Shotgun identification of proteins from rust spores. Phytopathology 95: S144.

Interpretive Summary:

Technical Abstract: We are interested in learning more about the biology of the economically important Phakopsora pachyrhizi, the causal agent of soybean rust. To accomplish this, we are beginning an in-depth study of the proteome of P. pachyrhizi using liquid chromatography tandem mass spectrometry (LC-MS/MS). LC-MS/MS offers the inherent benefit of providing protein sequence information that can be used to specifically identify and characterize proteins in a high-throughput fashion. This technology can be exploited to identify proteins that define particular life-cycle stages of the fungus. The knowledge of the proteins that form spores, germlings, appressoria, and haustoria may be used to define host-pathogen interactions or serve as targets for chemical inhibition of the fungus. Because of Select Agent containment restrictions, we have optimized our methodology using common bean rust Uromyces appendiculatus. A description of the spore proteome is presented. Comparative proteomics between the two fungi will follow.