Submitted to: International Poultry Forum Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/1/2004
Publication Date: 1/24/2005
Citation: Holt, P.S., Stone, H.D., Gast, R.K., Moore, R.W. 2005. Detection of specific antibody responses to salmonella enteritidis in lung wash samples from infected hens. International Poultry Forum Proceedings.
Technical Abstract: We have developed a simplified method for collecting lung lavage samples from adult chickens. Adult white leghorn hens previously infected with Salmonella enteritidis (SE) were euthanized using carbon dioxide inhalation and the tracheas were exteriorized. Narrow gauge tygon tubing (6/32") was inserted down the trachea into the lungs. Using a 30 ml syringe attached to the end of the tubing, a vacuum was applied to remove all air from the lung and then 10 ml of glycine buffer was slowly introduced into the lung and then slowly aspirated. Samples contaminated with blood were discarded. Antibody responses in the lung were determined via an ELISA using SE lipopolysaccharide as the solid phase antigen. A detectable IgA anti-SE response was observed in the lung lavage fluids one week post infection and these increased substantially over the next several weeks. These results indicate that a detectable immune response will develop in the lung of individuals intestinally infected with an enteric pathogen.