Submitted to: Phytochemistry
Publication Type: Peer reviewed journal
Publication Acceptance Date: 3/10/2004
Publication Date: 5/10/2004
Citation: Benedict, C.R., Martin, G.S., Liu, J., Puckhaber, L.S., Magill, C. 2004. Terpenoid aldehyde formation and lysigenous gland storage sites in cotton: Variant with mature glands but suppressed levels of terpenoid aldehydes. Phytochemistry. 64:1351-1359. Interpretive Summary: Cottonseed is a nutritious source of protein that is under-utilized because of the presence of a toxic compound called gossypol. It would be advantageous to develop cotton plants with seed that contained low levels of gossypol. To this end, cotton plants were developed that contained very low levels of gossypol by altering the genes in the plant. This technology could be used to produce cottonseed that has low levels of this toxic compound.
Technical Abstract: A new cotton variant with reduced levels of terpenoid aldehydes (sesquiterpenoids and sesterterpenoids (heliocides)) was isolated from the progeny of hemizygous cotton (Gossypium hirsutum cv. Coker 312) transformed with antisense (+)-delta-cadinene synthase cDNA. Southern analysis of leaf DNA digested with HINdIII, Pst or KpnI restriction endonucleases did not detect any antisense cdn1-C1 DNA in the genome of the variant. The gossypol content in the seed of the variant was markedly lower than in the seed of TI antisense plants. Eighty-nine percent of the variant seed had a 71.1% reduction in gossypol and the foliage of the variant plants showed a 70% reduction in gossypol and a 31% reduction in heliocides. Compared to non-transformed plants there was no reduction in the number of lysigenous glands in the seed of the variant. The cotton variant shows uncoupling of terpenoid aldehyde synthesis and gland formation. The cotton variant may have resulted from somaclonal variation occurring in the callus tissue during the transformation-regeneration process.