Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/1/2004
Publication Date: 7/20/2004
Citation: Hotchkiss, A.T., Manderson, K., Olano-Martin, E., Grace, W.E., Gibson, G.R., Rastall, R.A. 2004. Orange peel pectic oligosaccharide prebiotics with food and feed applications. Meeting Abstract.228th American Chemical Society National Meeting, Philadelphia, PA. August 22-26, 2004. CARB 38.
Technical Abstract: Prebiotics are non-digestible oligosaccharides which cause a limited species composition shift in the gut microflora by enhancing the growth of beneficial, health-promoting bacteria. Oligosaccharides derived from high-methoxy citrus pectin, low-methoxy apple pectin and orange peel enhanced the growth of Bifidobacteria and Lactobacilli while limiting the growth of pathogens using mixed fecal batch cultures and a FISH assay. Orange peel pectic oligosaccharides had rhamnogalacturonan composition. Pectic oligosaccharides were also able to interfere with the adhesion of verocytotoxins, derived from E. coli 0157:H7, in the human adenocarcinoma colonic cell line HT29 and stimulated apoptosis in these cells. Therefore, pectic oligosaccharides represent a new class of prebiotics with multiple health-promoting roles. Our efforts to find an inexpensive source of these pectic oligosaccharides in orange peel and sugar beet pulp, for food and feed applications, will be described.