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Title: IMMUNE RESPONSE TO A DNA VACCINE DERIVED FROM THE PASTEURELLA MULTOCIDA TOXIN GENE

Author
item Register, Karen
item Sacco, Randy
item Brockmeier, Susan

Submitted to: International Pig Veterinary Society (IPVS)
Publication Type: Proceedings
Publication Acceptance Date: 4/20/2004
Publication Date: 7/1/2004
Citation: Register, K.B., Sacco, R.E., Brockmeier, S. 2004. Immune response to a dna vaccine derived from the pasteurella multocida toxin gene. Pig Veterinary Society International Congress Proceedings. p. 200.

Interpretive Summary:

Technical Abstract: Pasteurella multocida is a widespread and costly pathogen responsible for both atrophic rhinitis and pneumonia in swine. The P. multocida toxin (PMT) is a primary virulence factor in atrophic rhinitis and a major protective immunogen. Technical difficulties and expense prevent the purification of PMT in amounts suitable for widespread use as a stand-alone vaccine or as an additive to existing vaccines. DNA vaccine technology may overcome these limitations and provide a practical solution for improving vaccine efficacy. In the present study, a PMT gene fragment truncated at the 5'end but predicted to encode the major immunogenic epitopes was cloned into 2 eukaryotic expression vectors and used to vaccinate mice. Humoral and cellular immune responses were analyzed to determine the suitability of the constructs for use as DNA vaccines.