Submitted to: Annual International Plant & Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/12/2003
Publication Date: 1/14/2003
Citation: DVORAK, J., ANDERSON, O.D., GILL, B.S., LUO, M., ZHANG, H., DEAL, K., LI, W., YOU, F., GU, Y.Q., MCGUIRE, P.E. ASSESSMENT OF THE INSULAR ORGANIZATION OF THE WHEAT D GENOME BY PHYSICAL MAPPING. Annual International Plant & Animal Genome Conference. 2003.
Technical Abstract: Physical map of the wheat genomes would greatly accelerate marker development, gene isolation, and studies on genome structure and evolution in this economically exceedingly important species and its relatives. To construct a physical map of the seven chromosomes of the genome of Ae. tauschii (the source of the D genome of common wheat), the following objectives have been either accomplished or are being pursued. Five genomic DNA libraries of Aegilops tauschii ssp. strangulata from Armenia (line AL 8/78-2-2) have been constructed. Three libraries are in a BAC vector (EcoRI, BamHI, and HindIII) and two are in a plant-transformation-competent BIBAC vector (BamHI and Hind III). A BAC fingerprinting procedure utilizing the commercially available SNaPshotTM (Applied Biosystems) was developed and automated. The overall goal is to fingerprint a total of 300,000 BAC clones and contig them. By the end of September, 2002, 70,000 BAC clones have been fingerprinted using four pairs of restriction enzymes and DNA fragments sized by ABI 3100 DNA sequencers. Wheat ESTs and other DNA markers mapped on Triticeae linkage or deletion maps are being hybridized with a subpopulation of 80,000 (~4 x) BAC clones, utilizing a two-dimensional pooling strategy.Close to 500 RFLP markers mapped on Triticeae genetic maps have been integrated into this BAC population by the end of September, 2002. The distribution of loci hybridizing with cDNA (EST) clones across chromosomes will be used to estimate gene densities across the D-genome chromosomes and to determine the location of gene islands. Databases of individual BAC fingerprints and contigs with integrated wheat markers (http://wheat.pw.usda.gov/PhysicalMapping) have been constructed and are publicly available.