Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/21/2004
Publication Date: 8/30/2004
Citation: Lam, N., Letchworth III, G.J. 2004. A derivative of bovine herpesvirus 1 (bhv-1) ul3.5 lacking the last forty amino acids inhibits replication of bhv-1. Archives of Virology (2004) 149:2295-2306.
Interpretive Summary: Bovine herpesvirus type 1 (BHV-1) is a common cause of disease in cattle. In this study, we found that bovine cells expressing a fragment of a BHV-1 gene called UL3.5 were 300- to 500-fold less susceptible to BHV-1 than control cells. Resistance was specific to BHV-1 as two other herpesviruses were fully infectious in these same cells.
Technical Abstract: BHV-1 UL3.5 is a tegument protein interacting with BHV-1 alpha-transinducing factor (alphaBTIF). In this report, we show that a derivative of UL3.5 lacking the last 40-amino acids (C40UL3.5) inhibited replication of BHV-1. C40UL3.5, when either transiently or stably expressed, inhibited alphaBTIF-mediated transactivation of a BHV-1 immediate-early promoter. Stable expression of C40UL3.5 in MDBK cells inhibited replication of BHV-1 300- to 500-fold in plaque assays. Expression of the viral immediate early gene BICP4 and the early protein gB were reduced and delayed in C40UL3.5-expressing cells infected with BHV-1. Cells expressing C40UL3.5 support replication of PrV and HSV-1 to normal levels. C40UL3.5 may be useful for constructing transgenic cattle resistant to infection by BHV-1.