|Blomberg, Le Ann|
Submitted to: The International Embryo Technology Society
Publication Type: Abstract Only
Publication Acceptance Date: 10/20/2002
Publication Date: 1/7/2003
Citation: Zuelke, K.A., Blomberg, L., Long, E.L., Sonstegard, T.S. Serial analysis of gene expression (sage) comparisons between day 11 and day 12 porcine embryos. THERIOGENOLOGY 59:435.
Technical Abstract: Mammalian embryo development represents a continuum of molecular and cellular interactions whereby early events dictate or influence subsequent developmental outcomes. Swine often exhibit high rates (>30%) of early embryonic mortality and the efficiency of producing swine embryos in vitro is poor compared to methods applied in other livestock species. The maternal recognition of pregnancy combined with the dramatic embryo elongation that occurs between Day 11 (D11) and Day 12 (D12) of gestation denote critical stages in porcine development. Serial Analysis of Gene Expression (SAGE) enables both qualitative and quantitative analysis of gene expression on a whole-transcriptome level (Velculescu VE et al., Science 1995; 270:484-487). We performed SAGE on in vivo-derived D11 and D12 porcine embryos to identify and characterize critical gene expression events that occur during and between these key stages of early porcine embryo development.We constructed SAGE libraries representing in vivo-derived D11 and D12 stage embryos as part of an ongoing project to develop and characterize gene expression profiles during comparative stages of in vivo and in vitro development of porcine embryos. Comparative analysis of these D11 and D12 embryo SAGE libraries confirmed significant levels of differential gene expression between these stages of porcine embryo development. SAGE enabled the identification of genes associated with putative functional pathways and demonstrated its potential utility facilitating the discovery of new genes in a poorly characterized system for future elucidation of the most critical differentially expressed genes.