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Title: POSITIONING CLASSICAL MARKERS OF THE SOYBEAN SSR MAP: AN UPDATE

Author
item SPECHT, JAMES - UNIVERSITY OF NEBRASKA
item GRAEF, G - UNIVERSITY OF NEBRASKA
item Cregan, Perry

Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: 10/22/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Soybean F2 populations, each segregating for just one of 100+ classical genes, were phenotyped to identify homozygous recessives (HRs). Using bulk-segregant analysis, a putative linkage of a SSR with each classical gene was identified. Most SSR-classical gene linkages were confirmed via allelic analysis (linked SSR plus nearby ones) of the 25+ individual F2 HRs. A maximum likelihood equation for partial (HR only) F2 data was used to compute recombination distances. Linkage of the classical gene with multiple SSRs was useful in positioning that gene on the SSR genetic map. Most classical gene linkage groups (LGs) had been assigned to their homologous SSR LGs in the Cregan et al 1999 paper, but the paired orientation was not clear in many cases. The SSR - classical linkage data helped resolve orientation ambiguity. The data also revealed that the Y11 - Df2 classical linkage group (LG), which was not yet positioned on the SSR map, belonged to SSR LG-F. The approach described here, if adapted by the Soybean Genetic Committee as a requirement before assignment of a (classical) gene symbol, would help point to which allelism tests to conduct. Moreover, the value of a classical gene is enhanced (relative to QTL studies) if its genetic map position is known.