Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/1/2001
Publication Date: 7/16/2001
Citation: Gast, R.K., Holt, P.S. 2001. Frequency and location of salmonella enteritidis contamination in eggs associated with various routes of experimental infection of laying hens. American Veterinary Medical Association Abstract.
Technical Abstract: Effective application of risk reduction practices such as refrigeration requires accurate information about the characteristics of Salmonella enteritidis (SE) contamination of eggs. Experimental infection models can be useful tools for understanding how SE is deposited in eggs and for testing potential control strategies. The present study compared the frequency and location of SE deposition in egg contents following experimental inoculation by three different routes. In two replicate trials, specific-pathogen-free laying hens were infected with an SE culture mixture prepared to optimize invasive behavior. Groups of hens received either an oral, aerosol, or intravenous dose of SE. Fecal shedding of SE and serum antibody responses to SE flagellin were monitored to characterize the experimental infections induced by the various routes of inoculation. Eggs laid during the first 4 weeks post-inoculation were cultured to detect tand enumerate SE in the yolk and albumen. The comparative patterns of deposition of SE in eggs indicated differences in the usefulness of the various experimental models.