|Quigley, Charles - Chuck|
Submitted to: Biennial Conference on Molecular and Cellular Biology of the Soybean
Publication Type: Abstract Only
Publication Acceptance Date: 8/14/2000
Publication Date: N/A
Technical Abstract: Soybean cultivars are described for purposes of Plant Variety Protection (PVP) by standard pigmentation and morphological traits. However, many commercial soybeans are indistinguishable based on these traits. A system based on DNA markers could provide unique DNA profiles of cultivars. Simple Sequence Repeat (SSR) allele size profiling is used in human forensics to provide unique DNA fingerprints of an individual. The purpose of the work presented here was to select a small set of SSR markers with maximum reliability and repeatability that would provide a high level of discriminatory power to distinguish soybean genotypes. A total of 48 fluorescently labelled SSR primer sets was used to amplify genomic DNA of the 35 ancestors of N. American soybeans as well as a diverse group of elite N. American soybean cultivars. Only loci with allele size ranges that showed no overlap in size over a series of analyses and in which adjacent alleles differed by at least three basepairs were maintained for further statistical analysis via a clustering procedure. Cluster analysis on the remaining loci resulted in the identification of a subset of 13 loci, from 12 different linkage groups, that easily produced unique SSR allele size profiles for each of the 66 elite N. American soybean cultivars. This set of 13 loci was used to characterize four independent sets of elite cultivars that were selected based upon identical maturity, morphological, and pigmentation traits. Based upon these analyses, all cultivars could be distinguished using the set of 13 selected SSR loci. This set of loci is proposed as a standard set for use in DNA profiling of soybean cultivars for purposes of obtaining PVP.