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ARS Home » Plains Area » El Reno, Oklahoma » Grazinglands Research Laboratory » Forage and Livestock Production Research » Research » Publications at this Location » Publication #111858

Title: EFFECT OF UNDEGRADABLE INTAKE PROTEIN SUPPLEMENTS, FORAGE PROTEIN LEVEL, AND INCUBATION TIME ON IN VITRO FERMENTATION

Author
item Appeddu, Lisa
item PETERSEN, M
item RICHARDS, J
item REMMENGA, M

Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: 7/1/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: High undegradable intake protein (UIP) supplements may interact with forage quality to affect ruminal fermentation. Two in vitro experiments investigated changes in OMD, ammonia and VFA production with UIP supplements (S) and forage (F) at 3,8,13%CP over time (T; 18 or 48 h). In Experiment 1, forage was incubated with no supplement (NS), wheat middlings(WM;23%CP),or WM+feather meal(FM) at 7,16,23%UIP(32,44,55%CP). In Experiment 2, NS, WM(17%CP),or WM+FM at 9,12,15%UIP (34%CP) was supplied. In Experiment 1, OMD was increased by S vs NS(33,40+.4%;P=.01) and F CP (31,40,45+.3%;P=.01). Ammonia was increased by UIP at 48 h(SxT;P=.01). Acetate:propionate decreased as F CP increased, but increased by NS and high UIP (SxF;P=.01). Total VFA increased as F CP increased, but decreased by UIP (SxF;P=.01). In Experiment 2, NS decreased OMD in lower CP forage(SxF;P=.02). Ammonia was lowest (P=.01) in NS and highest (P=.01) in WM+FM at 9%UIP. Acetate:propionate was decreased by higher WM and F CP(SxF;P=.01). Total VFA at 48 h was decreased (P=.01) by NS vs S (60vs75+1.6 mMol) and increased(P=.01) by F CP(68,69,79+1.3 mMol). A moderate protein supplement optimized in vitro forage fermentation, with high UIP having less benefits on high protein forage. Forage degradation was most increased in the first supplemental increment.