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Steven Joseph Klosterman
Crop Improvement and Protection Research
Research Molecular Biologist

Phone: (831) 755-2845
Fax: (831) 755-2814

1636 E ALISAL ST
SALINAS, CA 93905
Projects
Management of Pathogens for Strawberry and Vegetable Production Systems
In-House Appropriated (D)
  Accession Number: 431470
Epidemiology of Spinach Downy Mildew Pathogen Peronospora effusa
Non-Funded Cooperative Agreement (N)
  Accession Number: 436200
A Platform for Breeding Broad Genetic Resistance to Downy Mildew for Organic Spinach Production
Reimbursable Cooperative Agreement (R)
  Accession Number: 435569
Detection and Disease Forecasting for Downy Mildew Pathogens
Non-Assistance Cooperative Agreement (S)
  Accession Number: 431663
Detection, Epidemiology, and Control of Spinach Downy Mildew
Trust Fund Cooperative Agreement (T)
  Accession Number: 438116

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
RPA assay detection of spinach downy mildew in leaves ()
Klosterman, S.J., Anchieta, A.G. 2020. RPA assay detection of spinach downy mildew in leaves. Demonstration for the California Leafy Greens Research Program. Available: https://calgreens.org/
Genome sequence of Verticillium dahliae race 1 isolate VdLs.16 from lettuce Reprint Icon - (Peer Reviewed Journal)
Chen, J.-Y., Zhang, D.-D., Huang, J.-Q., Wang, D., Hao, S.-J., Li, R., Puri, K.D., Yang, L., Tong, B.-Z., Xiong, K.-X., Simko, I., Klosterman, S.J., Subbarao, K.V., Dai, X.-F. 2020. Genome sequence of Verticillium dahliae race 1 isolate VdLs.16 from lettuce. Molecular Plant-Microbe Interactions. https://doi.org/10.1094/MPMI-04-20-0103-A.
Sporangiospore viability and oospore production in the spinach downy mildew pathogen, Peronospora effusa Reprint Icon - (Peer Reviewed Journal)
Dhillon, B., Villarroel-Zeballos, M.I., Castroagudin, V.L., Bhattarai, G., Klosterman, S.J., Correll, J.C. 2020. Sporangiospore viability and oospore production in the spinach downy mildew pathogen, Peronospora effusa. Plant Disease. https://doi.org/10.1094/PDIS-02-20-0334-RE.
Transcriptional analyses of differential cultivars during resistant and susceptible interactions with Peronospora effusa, the causal agent of spinach downy mildew Reprint Icon - (Peer Reviewed Journal)
Kandel, S.L., Hulse-Kemp, A.M., Stoffel, K., Koike, S.T., Shi, A., Mou, B., Van Deynze, A., Klosterman, S.J. 2020. Transcriptional analyses of differential cultivars during resistant and susceptible interactions with Peronospora effusa, the causal agent of spinach downy mildew. Scientific Reports. 10:6719. https://doi.org/10.1038/s41598-020-63668-3.
Genome sequences of Verticillium dahliae defoliating strain XJ592 and nondefoliating strain XJ511 Reprint Icon - (Peer Reviewed Journal)
Li, H., Dai, J., Qin, J., Shang, W., Chen, J., Zhang, L., Dai, X., Klosterman, S.J., Xu, X., Subbarao, K.V., Fan, S., Hu, X. 2020. Genome sequences of Verticillium dahliae defoliating strain XJ592 and nondefoliating strain XJ511. Molecular Plant-Microbe Interactions. 33(4):565-568. https://doi.org/10.1094/MPMI-11-19-0320-A.
The Arabidopsis senescence associated gene 13 regulates dark-induced senescence and plays contrasting roles in the defense against bacterial and fungal pathogens Reprint Icon - (Peer Reviewed Journal)
Dhar, N., Caruana, J., Erdem, I., Subbarao, K., Klosterman, S.J., Raina, R. 2020. The Arabidopsis senescence associated gene 13 regulates dark-induced senescence and plays contrasting roles in the defense against bacterial and fungal pathogens. Molecular Plant-Microbe Interactions. 33(5):754-766. https://doi.org/10.1094/MPMI-11-19-0329-R.
The bZIP transcription factor VdAtf1 regulates virulence by mediating nitrogen metabolism in Verticillium dahliae Reprint Icon - (Peer Reviewed Journal)
Tang, C., Li, T., Klosterman, S.J., Tian, C., Wang, Y. 2020. The bZIP transcription factor VdAtf1 regulates virulence by mediating nitrogen metabolism in Verticillium dahliae. New Phytologist. 226(5):1461-1479. https://doi.org/10.1111/nph.16481.
Functional analyses of small secreted cysteine-rich proteins identified candidate effectors in Verticillium dahliae Reprint Icon - (Peer Reviewed Journal)
Wang, D., Tian, L., Zhang, D.-D., Song, J., Song, S.-S., Yin, C.-M., Zhou, L., Liu, Y., Wang, B.-L., Kong, Z.-Q., Klosterman, S.J., Li, J.-J., Wang, J., Li, T.-G., Adamu, S., Subbarao, K.V., Chen, J.-Y., Dai, X.-F. 2020. Functional analyses of small secreted cysteine-rich proteins identified candidate effectors in Verticillium dahliae. Molecular Plant Pathology. 21(5):667–685. https://doi.org/10.1111/mpp.12921.
The Verticillium dahliae Sho1-MAPK pathway regulates melanin biosynthesis and is required for cotton infection Reprint Icon - (Peer Reviewed Journal)
Li, J.-J., Zhou, L., Yin, C.-M., Zhang, D.-D., Klosterman, S.J., Wang, B.-L., Song, J., Wang, D., Hu, X.-P., Subbarao, K.V., Chen, J.-Y., Dai, X.-F. 2019. The Verticillium dahliae Sho1-MAPK pathway regulates melanin biosynthesis and is required for cotton infection. Environmental Microbiology. 21(12):4852-4874. https://doi.org/10.1111/1462-2920.14846.
Early detection, epidemiology, and control of spinach downy mildew ()
Klosterman, S.J. 2019. Early detection, epidemiology, and control of spinach downy mildew. California Leafy Greens Research Program Mid-Year Meeting, October 8, 2019, Salinas, California.
Investigation of a spinach seed treatment, and genetic analyses of the downy mildew-spinach interaction ()
Klosterman, S.J. 2019. Investigation of a spinach seed treatment, and genetic analyses of the downy mildew-spinach interaction. California Seed Association Mid-Year Meeting, October 7, 2019, Monterey, California.
Measurements of aerial spore load by qPCR facilitates lettuce downy mildew risk advisement Reprint Icon - (Peer Reviewed Journal)
Dhar, N., Mamo, B.E., Subbarao, K.V., Koike, S.T., Fox, A., Anchieta, A.G., Klosterman, S.J. 2019. Measurements of aerial spore load by qPCR facilitates lettuce downy mildew risk advisement. Plant Disease. 104(1):82-93. https://doi.org/10.1094/PDIS-03-19-0441-RE.
Evaluation of biopesticides for managing downy mildew of spinach in organic production systems in 2017 and 2018 - (Peer Reviewed Journal)
Kandel, S.L., Subbarao, K.V., Shi, A., Mou, B., Klosterman, S.J. 2019. Evaluation of biopesticides for managing downy mildew of spinach in organic production systems in 2017 and 2018. Plant Disease Management Reports. 13:V171.
Proteome and metabolome analyses reveal differential responses in tomato-Verticillium dahliae-interactions Reprint Icon - (Peer Reviewed Journal)
Hu, X., Puri, K.D., Gurung, S., Klosterman, S.J., Wallis, C.M., Britton, M., Durbin-Johnson, B., Phinney, B., Salemi, M., Short, D.P.G., Subbarao, K.V. 2019. Proteome and metabolome analyses reveal differential responses in tomato-Verticillium dahliae-interactions. Journal of Proteomics. 207:103449. https://doi.org/10.1016/j.jprot.2019.103449.
Two Verticillium dahliae MAPKKKs, VdSsk2 and VdSte11, have distinct roles in pathogenicity, microsclerotial formation, and stress adaptation Reprint Icon - (Peer Reviewed Journal)
Yu, J., Li, T., Tian, L., Tang, C., Klosterman, S.J., Tian, C., Wang, Y. 2019. Two Verticillium dahliae MAPKKKs, VdSsk2 and VdSte11, have distinct roles in pathogenicity, microsclerotial formation, and stress adaptation. mSphere. 4(4). https://doi.org/10.1128/mSphere.00426-19.
Insights into VdCmr1-mediated protection against high temperature stress and UV irradiation in Verticillium dahliae Reprint Icon - (Peer Reviewed Journal)
Fang, Y., Klosterman, S.J., Tian, C., Wang, Y. 2019. Insights into VdCmr1-mediated protection against high temperature stress and UV irradiation in Verticillium dahliae. Environmental Microbiology. 21(8):2977-2996. https://doi.org/10.1111/1462-2920.14695.
Improved detection methods for the spinach downy mildew pathogen, Peronospora effusa - (Abstract Only)
Batista Da Silva, M., Choi, Y., Martin, F.N., Osorio Marin, J., Garcia-Llanos, A., Van Deynze, A., Klosterman, S.J. 2019. Improved detection methods for the spinach downy mildew pathogen, Peronospora effusa. American Phytopathological Society Annual Meeting, August 3-7, 2019, Cleveland, Ohio.
Comparative genomics of downy mildews - (Abstract Only)
Fletcher, K., Martin, F.N., Rivera, Y., Gil, J., Wood, K., Dela Cueva, F., Kandel, S.L., Luster, D.G., Magill, C., Sharma, R., Klosterman, S.J., Abad, G., Michelmore, R. 2019. Comparative genomics of downy mildews. Oomycete Molecular Genetics Network Conference, July 10-12, 2019, Oban, Scotland.
Arabidopsis defense mutant ndr1-1 displays accelerated development and early flowering mediated by the hormone gibberellic acid Reprint Icon - (Peer Reviewed Journal)
Dhar, N., Short, D.P., Mamo, B.E., Corrion, A.J., Wai, C.M., Anchieta, A.G., Van Buren, R., Day, B., Ajwa, H., Subbarao, K.V., Klosterman, S.J. 2019. Arabidopsis defense mutant ndr1-1 displays accelerated development and early flowering mediated by the hormone gibberellic acid. Plant Science. 285:200-213. https://doi.org/10.1016/j.plantsci.2019.04.006.
Hormone signaling, growth, and defense response - Arabidopsis ndr1-1 takes center stage - (Abstract Only)
Dhar, N., Short, D.P., Mamo, B.E., Anchieta, A.G., Corrion, A., Day, B., Ajwa, H., McCreight, J.D., Casteel, C., Subbarao, K.V., Klosterman, S.J. 2019. Hormone signaling, growth, and defense response - Arabidopsis ndr1-1 takes center stage. American Society of Plant Biologists Annual Meeting, August 4-7, 2019, San Jose, California.
Transcriptional analysis of spinach cultivars during resistant and susceptible interactions with the downy mildew pathogen, Peronospora effusa - (Abstract Only)
Kandel, S.L., Hulse-Kemp, A., Mou, B., Van Deynze, A., Klosterman, S.J. 2019. Transcriptional analysis of spinach cultivars during resistant and susceptible interactions with the downy mildew pathogen, Peronospora effusa. American Phytopathological Society Annual Meeting, August 3-7, 2019, Cleveland, Ohio. Available: https://apsnet.confex.com/apsnet/2019/meetingapp.cgi/Paper/14429.
The polyketide synthase VdPKS1 and the transcription factor VdCmr1 are required for pigment production and UV protection in Verticillium dahliae - (Abstract Only)
Wang, Y., Hu, X., Fang, Y., Anchieta, A.G., Goldman, P.H., Hernandez, G., Klosterman, S.J. 2019. The polyketide synthase VdPKS1 and the transcription factor VdCmr1 are required for pigment production and UV protection in Verticillium dahliae. American Phytopathological Society Annual Meeting, August 3-7, 2019, Cleveland, Ohio. Available: https://apsnet.confex.com/apsnet/2019/meetingapp.cgi/Paper/13416.
The Gossypium hirsutum TIR-NBS-LRR gene GhDSC1 mediates resistance against Verticillium wilt Reprint Icon - (Peer Reviewed Journal)
Li, T., Wang, B., Yin, C., Zhang, D., Wang, D., Song, J., Zhou, L., Kong, Z., Klosterman, S.J., Li, J., Adamu, S., Liu, T., Subbarao, K.V., Chen, J., Dai, X. 2019. The Gossypium hirsutum TIR-NBS-LRR gene GhDSC1 mediates resistance against Verticillium wilt. Molecular Plant Pathology. 20(6):857-876. https://doi.org/10.1111/mpp.12797.
Population genomics demystifies the defoliation phenotype in the plant pathogen Verticillium dahliae Reprint Icon - (Peer Reviewed Journal)
Zhang, D., Wang, J., Wang, D., Kong, Z., Zhou, L., Zhang, G., Gui, Y., Li, J., Huang, J., Wang, B., Liu, C., Yin, C., Li, R., Li, T., Wang, J., Short, D.P., Klosterman, S.J., Bostock, R.M., Subbarao, K.V., Chen, J., Dai, X. 2019. Population genomics demystifies the defoliation phenotype in the plant pathogen Verticillium dahliae. New Phytologist. 222(2):1012-1029. https://doi.org/10.1111/nph.15672.
Genome-wide transcriptome profiles reveal how Bacillus subtilis lipopeptides inhibit microsclerotia formation in Verticillium dahliae Reprint Icon - (Peer Reviewed Journal)
Yu, D., Fang, Y., Tang, C., Klosterman, S.J., Tian, C., Wang, Y. 2018. Genome-wide transcriptome profiles reveal how Bacillus subtilis lipopeptides inhibit microsclerotia formation in Verticillium dahliae. Molecular Plant-Microbe Interactions. 32(5):622-634. https://doi.org/10.1094/MPMI-08-18-0233-R.
Spinach downy mildew: Advances in our understanding of the disease cycle and prospects for disease management Reprint Icon - (Peer Reviewed Journal)
Kandel, S.L., Mou, B., Shishkoff, N., Shi, A., Subbarao, K.V., Klosterman, S.J. 2019. Spinach downy mildew: Advances in our understanding of the disease cycle and prospects for disease management. Plant Disease. 103:791-803. https://doi.org/10.1094/PDIS-10-18-1720-FE.
Comparative genomics of downy mildews reveals potential adaptations to biotrophy Reprint Icon - (Peer Reviewed Journal)
Fletcher, K., Klosterman, S.J., Derevnina, L., Martin, F.N., Bertier, L.D., Koike, S.T., Chin-Wo-Reyes, S., Mou, B., Michelmore, R. 2018. Comparative genomics of downy mildews reveals potential adaptations to biotrophy. BMC Genomics. 19:851. https://doi.org/10.1186/s12864-018-5214-8.
The transcription factor VdHapX controls iron homeostasis and is crucial for virulence in the vascular pathogen Verticillium dahliae Reprint Icon - (Peer Reviewed Journal)
Wang, Y., Deng, C., Tian, L., Xiong, D., Tian, C., Klosterman, S.J. 2018. The transcription factor VdHapX controls iron homeostasis and is crucial for virulence in the vascular pathogen Verticillium dahliae. mSphere. 3(5):e00400-18. https://doi.org/10.1128/mSphere.00400-18.
Genome-wide identification and functional analyses of the CRK gene family reveals that GbCRK18 confers Verticillium wilt resistance in Gossypium barbadense Reprint Icon - (Peer Reviewed Journal)
Li, T.-G., Zhang, D.-D., Zhou, L., Kong, Z.-Q., Hussaini, A.S., Wang, D., Li, J.-J., Short, D.P.G., Dhar, N., Klosterman, S.J., Wang, B.L., Yin, C.-M., Subbarao, K.V., Chen, J.-Y., Dai, X.-F. 2018. Genome-wide identification and functional analyses of the CRK gene family reveals that GbCRK18 confers Verticillium wilt resistance in Gossypium barbadense. Frontiers in Plant Science. 9:1266. https://doi.org/10.3389/fpls.2018.01266.
Detection of latent Peronospora effusa infections in spinach Reprint Icon - (Peer Reviewed Journal)
Subbarao, C.S., Anchieta, A.G., Ochoa, L., Dhar, N., Kunjeti, S.G., Subbarao, K.V., Klosterman, S.J. 2018. Detection of latent Peronospora effusa infections in spinach. Plant Disease. https://doi.org/10.1094/PDIS-12-17-1956-RE.
Comparative genomics of downy mildews - (Abstract Only)
Michelmore, R., Fletcher, K., Martin, F.N., Gil, J., Zhang, L., Wood, K., Kenefick, A., Dela Cueva, F., Luster, D.G., Rivera, Y., Magill, C., Sharma, R., Klosterman, S.J., Zhang, Y., Abad, G., Reyes-Chin-Wo, S. 2018. Comparative genomics of downy mildews. International Congress of Plant Pathology, 6th International Oomycetes Workshop: Phytophthora, Pythium, Downy Mildews and Related Genera, July 28, 2018, Boston, Massachusetts.
Transcription factor VdCmr1 is required for pigment production, protection from UV irradiation, and regulates expression of melanin biosynthetic genes in Verticillium dahliae Reprint Icon - (Peer Reviewed Journal)
Wang, Y., Hu, X., Fang, Y., Anchieta, A.G., Goldman, P.H., Hernandez, G., Klosterman, S.J. 2018. Transcription factor VdCmr1 is required for pigment production, protection from UV irradiation, and regulates expression of melanin biosynthetic genes in Verticillium dahliae. Microbiology. 164(4):685-696. https://doi.org/10.1099/mic.0.000633.
SNARE-encoding genes VdSec22 and VdSso1 mediate protein secretion required for full virulence in Verticillium dahliae Reprint Icon - (Peer Reviewed Journal)
Wang, J., Tian, L., Zhang, D., Short, D.P., Zhou, L., Song, S., Liu, Y., Wang, D., Kong, Z., Cui, W., Ma, X., Klosterman, S.J., Subbarao, K.V., Chen, J., Dai, X. 2018. SNARE-encoding genes VdSec22 and VdSso1 mediate protein secretion required for full virulence in Verticillium dahliae. Molecular Plant-Microbe Interactions. 32(6):651-664. http://doi.org/10.1094/MPMI-12-17-0289-R.
Heterologous expression of the cotton NBS-LRR gene GbaNA1 enhances Verticillium wilt resistance in Arabidopsis Reprint Icon - (Peer Reviewed Journal)
Li, N.Y., Zhou, L., Zhang, D.-D., Klosterman, S.J., Li, T.-G., Gui, Y.J., Kong, Z.-Q., Ma, X.-F., Short, D.P.G., Zhang, W.-Q., Li, J.-J., Subbarao, K.V., Chen, J.-Y., Dai, X.-F. 2018. Heterologous expression of the cotton NBS-LRR gene GbaNA1 enhances Verticillium wilt resistance in Arabidopsis. Frontiers in Plant Science. https://doi.org/10.3389/fpls.2018.00119.
Characterization of two homeodomain transcription factors with critical but distinct roles in virulence in the vascular pathogen Verticillium dahliae - (Peer Reviewed Journal)
Sarmiento-Villamil, J.L., Prieto, P., Klosterman, S.J., Garcia-Pedrajas, M.D. 2017. Characterization of two homeodomain transcription factors with critical but distinct roles in virulence in the vascular pathogen Verticillium dahliae. Molecular Plant Pathology. http://doi.org/10.1111/mpp.12584.
A framework for optimizing phytosanitary thresholds in seed systems Reprint Icon - (Peer Reviewed Journal)
Choudhury, R.A., Garrett, K.A., Klosterman, S.J., Subbarao, K.V., Mcroberts, N. 2017. A framework for optimizing phytosanitary thresholds in seed systems. Phytopathology. 107(10):1219-1228. https://doi.org/10.1094/PHYTO-04-17-0131-FI.
The endochitinase VDECH from Verticillium dahliae inhibits spore germination and activates plant defense responses - (Peer Reviewed Journal)
Cheng, X., Zhao, L., Klosterman, S.J., Feng, H., Feng, Z., Wei, F., Shi, Y., Li, Z., Zhu, H. 2017. The endochitinase VDECH from Verticillium dahliae inhibits spore germination and activates plant defense responses. Plant Science. 259:12–23.
The Vayg1 gene controls microsclerotia development and melanin production in Verticillium dahliae - (Peer Reviewed Journal)
Fan, R., Klosterman, S.J., Wang, C., Subbarao, K.V., Xu, X., Shang, W., Hu, X. 2017. The Vayg1 gene controls microsclerotia development and melanin production in Verticillium dahliae. Fungal Genetics and Biology. 98:1–11.
Lettuce and spinach breeding - (Proceedings)
Simko, I., McCreight, J.D., Mou, B., Klosterman, S.J. 2016. Lettuce and spinach breeding. California Leafy Greens Research Program Mid-Year Meeting. October 4, 2016, Salinas, California.
Disease management in the genomics era - Summaries of focus issue papers ()
Klosterman, S.J., Rollins, J.R., Sudarshana, M.R., Vinatzer, B.A. 2016. Disease management in the genomics era - Summaries of focus issue papers. Phytopathology. 106:1068-1070.
Spatiotemporal patterns in the airborne dispersal of spinach downy mildew - (Peer Reviewed Journal)
Choudhury, R.A., Koike, S.T., Fox, A., Anchieta, A.G., Subbarao, K.V., Klosterman, S.J., McRoberts, N. 2017. Spatiotemporal patterns in the airborne dispersal of spinach downy mildew. Phytopathology. 107(1):50-58.
Season-long dynamics of spinach downy mildew determined by spore trapping and disease - (Peer Reviewed Journal)
Choudhury, R.A., Koike, S.T., Fox, A.D., Anchieta, A.G., Subbarao, K.V., Klosterman, S.J., McRoberts, N. 2016. Season-long dynamics of spinach downy mildew determined by spore trapping and disease. Phytopathology. 106:1311-1318.
Detection and quantification of Bremia lactucae by spore trapping and quantitative PCR - (Peer Reviewed Journal)
Kunjeti, S.G., Anchieta, A.G., Martin, F.N., Choi, Y.-J., Thines, M., Michelmore, R.W., Koike, S.T., Tsuchida, C., Mahaffee, W.F., Subbarao, K.V., Klosterman, S.J. 2016. Detection and quantification of Bremia lactucae by spore trapping and quantitative PCR. Phytopathology. 106:1426-1437.
Spinach downy mildew – Threat, prevention and control ()
Klosterman, S.J. 2016. Spinach downy mildew – Threat, prevention and control. Progressive Crop Consultant. 1:12-15.
The mitogen-activated protein kinase gene, VdHog1, regulates osmotic stress response, microsclerotia formation and virulence in Verticillium dahliae - (Peer Reviewed Journal)
Wang, Y., Tian, L., Xiong, D., Klosterman, S.J., Xiao, S., Tian, C. 2016. The mitogen-activated protein kinase gene, VdHog1, regulates osmotic stress response, microsclerotia formation and virulence in Verticillium dahliae. Fungal Genetics and Biology. 88:13–23.
VdCYC8, encoding CYC8 glucose repression mediator protein, is required for microsclerotia formation and full virulence in Verticillium dahliae - (Peer Reviewed Journal)
Li, Z., Liu, Y., Feng, Z., Feng, H., Klosterman, S.J., Zhou, F., Zhao, L., Shi, Y., Zhu, H. 2015. VdCYC8, encoding CYC8 glucose repression mediator protein, is required for microsclerotia formation and full virulence in Verticillium dahliae. PLoS One. 10(12):e0144020. doi: 10.1371/journal.pone.0144020.
Characterization of spinach germplasm for resistance against two races of Verticillium dahliae - (Peer Reviewed Journal)
Mou, B., Klosterman, S.J., Anchieta, A.G., Wood, E.M., Subbarao, K. 2015. Characterization of spinach germplasm for resistance against two races of Verticillium dahliae. HortScience. 50:1631-1635.
Plasmolysis and vital staining reveal viable oospores of Peronospora effusa in spinach seed lots - (Peer Reviewed Journal)
Kunjeti, S.G., Anchieta, A.G., Subbarao, K.V., Koike, S.T., Klosterman, S.J. 2016. Plasmolysis and vital staining reveal viable oospores of Peronospora effusa in spinach seed lots. Plant Disease. 100:59-65.
Focus issue articles on emerging and re-emerging plant diseases ()
Subbarao, K.V., Sundin, G.W., Klosterman, S.J. 2015. Focus issue articles on emerging and re-emerging plant diseases. Phytopathology. 105:852-854.
Genomics spurs rapid advances in our understanding of the biology of vascular wilt pathogens in the genus Verticillium - (Peer Reviewed Journal)
Klimes, A., Dobinson, K.F., Thomma, B.P.H.J., Klosterman, S.J. 2015. Genomics spurs rapid advances in our understanding of the biology of vascular wilt pathogens in the genus Verticillium. Annual Review of Phytopathology. 53:181-198.
Repetitive elements, architects of genomic variation in Verticillium - (Abstract Only)
Dobinson, K.F., Klosterman, S.J., Anchieta, A.G., Li, Z., Amyotte, S., Ma, L. 2015. Repetitive elements, architects of genomic variation in Verticillium. Phytopathology. Available: http://www.apsnet.org/meetings/Documents/2015_meeting_abstracts/aps2015abO41.htm.
Analysis of a MULE-cyanide hydratase gene fusion in Verticillium dahliae - (Abstract Only)
Li, Z., Anchieta, A.G., Klosterman, S.J. 2015. Analysis of a MULE-cyanide hydratase gene fusion in Verticillium dahliae. Phytopathology. Available: http://www.apsnet.org/meetings/Documents/2015_meeting_abstracts/aps2015abP74.htm.
Detection of spinach downy mildew during latent infection - (Abstract Only)
Subbarao, C., Anchieta, A.G., Subbarao, K.V., Klosterman, S.J. 2015. Detection of spinach downy mildew during latent infection. Phytopathology. Available: http://www.apsnet.org/meetings/Documents/2015_meeting_abstracts/aps2015abP331.htm.
Epidemiology and control of spinach downy mildew in coastal California - (Abstract Only)
Choudhury, R., Koike, S.T., Subbarao, K.V., Klosterman, S.J., McRoberts, N. 2015. Epidemiology and control of spinach downy mildew in coastal California. Phytopathology. Available: http://www.apsnet.org/meetings/Documents/2015_meeting_abstracts/aps2015abO94.htm.
Development of an assay for rapid detection of the lettuce downy mildew pathogen, Bremia lactucae - (Abstract Only)
Kunjeti, S., Choi, Y., Anchieta, A.G., Thines, M., Michelmore, R., Koike, S.T., Tsuchida, C., Martin, F.N., Subbarao, K.V., Klosterman, S.J. 2015. Development of an assay for rapid detection of the lettuce downy mildew pathogen, Bremia lactucae. Phytopathology. Available: http://www.apsnet.org/meetings/Documents/2015_meeting_abstracts/aps2015abP42.htm.
Multi-locus tree and species tree approaches toward resolving a complex clade of downy mildews (Straminipila, Oomycota), including pathogens of beet and spinach - (Peer Reviewed Journal)
Choi, Y.-J., Klosterman, S.J., Kummer, V., Voglmayr, H., Shin, H.-D., Thines, M. 2015. Multi-locus tree and species tree approaches toward resolving a complex clade of downy mildews (Straminipila, Oomycota), including pathogens of beet and spinach. Molecular Phylogenetics and Evolution. 86:24–34.
The Downy Mildews: so many genomes, so little time - (Abstract Only)
Derevnina, L., Chin-Wo-Reyes, S., Martin, F.N., Wood, K., Froenicke, L., Wong, J., Gil, J., Klosterman, S.J., Spring, O., Sharma, R., Vashney, R., Magill, C., Michelmore, R. 2015. The Downy Mildews: So many genomes, so little time. Plant and Animal Genome XXIII (PAG) Conference. W436.
Frequency of Verticillium species in commercial spinach fields and transmission of V. dahliae from spinach to subsequent lettuce crops - (Peer Reviewed Journal)
Short, D.P., Gurung, S., Koike, S.T., Klosterman, S.J., Subbarao, K.V. 2015. Frequency of Verticillium species in commercial spinach fields and transmission of V. dahliae from spinach to subsequent lettuce crops. Phytopathology. 105:80-90.
Verticillium alfalfae and V. dahliae, agents of Verticillium wilt diseases ()
Inderbitzin, P., Thomma, B.P.H.J., Klosterman, S.J., Subbarao, K.V. 2014. Verticillium alfalfae and V. dahliae, agents of Verticillium wilt diseases. In: Dean, R.A., Lichens-Park, A., Kole, C., editors. Genomics of Plant-Associated Fungi and Oomycetes: Dicot Pathogens. Berlin Heidelberg, Germany: Springer-Verlag. p. 65-97.
Coupling spore traps and quantitative PCR assays for detection of the downy mildew pathogens of spinach (Peronospora effusa) and beet (Peronospora schachtii) - (Peer Reviewed Journal)
Klosterman, S.J., Anchieta, A.G., Mcroberts, N., Koike, S.T., Subbarao, K.V., Voglmayr, H., Choi, Y., Thines, M., Martin, F.N. 2014. Coupling spore traps and quantitative PCR assays for detection of the downy mildew pathogens of spinach (Peronospora effusa) and beet (Peronospora schachtii). Phytopathology. 104:1349-1359.
Deep mRNA sequencing reveals stage-specific transcriptome alterations during microsclerotia development in the smoke tree vascular wilt pathogen, Verticillium dahliae - (Peer Reviewed Journal)
Xiong, D., Wang, Y., Ma, J., Klosterman, S.J., Xiao, S., Tian, C. 2014. Deep mRNA sequencing reveals stage-specific transcriptome alterations during microsclerotia development in the smoke tree vascular wilt pathogen, Verticillium dahliae. BMC Genomics. 15:324.
Detection of the downy mildew pathogens of spinach (Peronospora effusa) and beet (P. schachtii) using spore traps and quantitative PCR assays - (Abstract Only)
Klosterman, S.J., Anchieta, A.G., Mcroberts, N., Koike, S.T., Subbarao, K.V., Voglmayr, H., Choi, Y., Thines, M., Martin, F.N. 2014. Detection of the downy mildew pathogens of spinach (Peronospora effusa) and beet (P. schachtii) using spore traps and quantitative PCR assays [abstract]. American Phytopathological Society. Available: http://www.apsnet.org/meetings/Documents/2014_meeting_abstracts/aps2014abO50.htm.
RNA-seq analyses of gene expression in the microsclerotia of Verticillium dahliae - (Peer Reviewed Journal)
Duressa, D., Anchieta, A.G., Chen, D., Klimes, A., Garcia-Pedrajas, M.D., Dobinson, K.F., Klosterman, S.J. 2013. RNA-seq analyses of gene expression in the microsclerotia of Verticillium dahliae. Biomed Central (BMC) Genomics. DOI: 10.1186/1471-2164-14-607.
High throughput analysis of gene expression in microsclerotia of Verticillium dahliae - (Abstract Only)
Klosterman, S.J., Duressa, D., Anchieta, A.G., Chen, D., Klimes, A., Garcia-Pedrajas, M.D., Dobinson, K.F. 2013. High throughput analysis of gene expression in microsclerotia of Verticillium dahliae. In: Koopmann, B., von Tiedemann, A., editors. Proceedings of the 11th International Verticillium Symposium. German Phytomedical Society, DPG-Verlag. p. 32.
Real-time PCR and spore trap-based detection of the downy mildew pathogen, Peronospora effusa - (Abstract Only)
Klosterman, S.J., McRoberts, N., Koike, S.T., Subbarao, K.V., Voglmayr, H., Thines, M., Martin, F.N. 2013. Real-time PCR and spore trap-based detection of the downy mildew pathogen, Peronospora effusa. Acta Phytopathologica Sinica. 43 (supplement):297.
Detection and quantification of Verticillium dahliae in spinach seed - (Abstract Only)
Duressa, D., Rauscher, G.M., Koike, S.T., Mou, B., Hayes, R.J., Maruthachalam, K., Subbarao, K.V., Klosterman, S.J. 2013. Detection and quantification of Verticillium dahliae in spinach seed. Acta Phytopathologica Sinica. 43 (supplement):245.
RNA-seq analyses of gene expression in the microsclerotia of Verticillium dahliae - (Abstract Only)
Duressa, D., Anchieta, A.G., Chen, D., Klimes, A., Dobinson, K.F., Garcia-Pedrajas, M., Klosterman, S.J. 2013. RNA-seq analyses of gene expression in the microsclerotia of Verticillium dahliae. Fungal Genetics Conference Proceedings. p. 194.
Colonization of spinach by Verticillium dahliae and effects of pathogen localization on the efficacy of seed treatments - (Peer Reviewed Journal)
Maruthachalam, K., Klosterman, S.J., Anchieta, A.G., Mou, B., Subbarao, K.V. 2013. Colonization of spinach by Verticillium dahliae and effects of pathogen localization on the efficacy of seed treatments. Phytopathology. 103:268-280.
Transposable elements in phytopathogenic Verticillium spp.: insights into genome evolution and inter- and intra-specific diversification - (Peer Reviewed Journal)
Amyotte, S.G., Tan, X., Pennerman, K., Del Mar Jimenez-Gasco, M., Klosterman, S.J., Ma, L., Dobinson, K.F., Veronese, P. 2012. Transposable elements in phytopathogenic Verticillium spp.: insights into genome evolution and inter- and intra-specific diversification. Biomed Central (BMC) Genomics. 13:314.
A real-time PCR assay for detection and quantification of verticillium dahliae in spinach seed - (Peer Reviewed Journal)
Duressa, D.O., Rauscher, G.M., Koike, S.T., Mou, B., Hayes, R.J., Maruthachalam, K., Subbarao, K.V., Klosterman, S.J. 2012. A real-time PCR assay for detection and quantification of verticillium dahliae in spinach seed. Phytopathology. 102:443-451.
Selection for resistance to Verticillium wilt caused by race 2 isolates of Verticillium dahliae in accessions of lettuce (Lactuca sativa L.). - (Peer Reviewed Journal)
Hayes, R.J., Maruthachalam, K., Vallad, G.E., Klosterman, S.J., Subbarao, K.V. 2011. Selection for resistance to Verticillium wilt caused by race 2 isolates of Verticillium dahliae in accessions of lettuce (Lactuca sativa L.).HortScience. 46(2):201-206.
Analysis of Verticillium dahliae suggests a lack of correlation between genotypic diversity and virulence phenotypes. - (Peer Reviewed Journal)
Atallah, Z.K., Maruthachalam, K.K., Vallad, G.E., Davis, M., Klosterman, S.J., Subbarao, K.V. 2011. Analysis of Verticillium dahliae suggests a lack of correlation between genotypic diversity and virulence phenotypes. Plant Disease. 95:1224-1232.
Verticillium comparative genomics yields insights into niche adaptation by plant vascular wilt pathogens. - (Abstract Only)
Klosterman, S.J., Subbarao, K.V., Kang, S., Veronese, P., Gold, S.E., Thomma, B., Chen, Z., Henrissat, B., Lee, Y., Park, J., Garcia-Pedrajas, M.D., Barbara, D., Anchieta, A.G., Jonge, R., Santhanam, P., Maruthachalam, K., Atallah, Z.K., Amyotte, S., Paz, Z., Inderbitzin, P., Hayes, R.J., Heiman, D., Young, S., Zeng, Q., Engels, R., Galagan, J., Cuomo, C., Dobinson, K.F., Ma, L. 2011. Verticillium comparative genomics yields insights into niche adaptation by plant vascular wilt pathogens. Phytopathology. 101:S208.
Colonization of spinach (Spinacia oleracea L.) by GFP-tagged verticillium dahliae. - (Abstract Only)
Maruthachalam, K., Klosterman, S.J., Subbarao, K.V. 2011. Colonization of spinach (Spinacia oleracea L.) by GFP-tagged verticillium dahliae. Phytopathology. 101: S115.
Development of a qPCR assay for quantification of verticillium dahliae in spinach seed. - (Abstract Only)
Duressa, D.O., Rauscher, G.M., Mou, B., Hayes, R.J., Koike, S.T., Maruthachalam, K., Subbarao, K.V., Klosterman, S.J. 2011. Development of a qPCR assay for quantification of verticillium dahliae in spinach seed.Phytopathology. 101:S46.
SSH reveals a linkage between a senescence-associated protease and Verticillium wilt symptom development in lettuce (Lactuca sativa) - (Peer Reviewed Journal)
Klosterman, S.J., Anchieta, A.G., Garcia-Pedrajas, M.D., Maruthachalam, K., Hayes, R.J., Subbarao, K.V. 2011. SSH reveals a linkage between a senescence-associated protease and Verticillium wilt symptom development in lettuce (Lactuca sativa). Physiological and Molecular Plant Pathology. 76:48-58.
Comparative genomics yields insights into niche adaptation of plant vascular wilt pathogens. - (Peer Reviewed Journal)
Klosterman, S.J., Subbarao, K.V., Kang, S., Veronese, P., Gold, S.E., Thomma, B.P.H.J, Chen, Z., Henrissat, B., Lee, Y., Park, J., Garcia-Pedrajas, M.D., Dez, B.J., Anchieta, A.G., De Jonge, R., Parthasarathy, S., Maruthachalam, K., Atallah, Z.K., Amyotte, S., Paz, Z., Inderbitizin, P., Hayes, R.J., Heiman, D.I., Young, S., Zeng, Q., Engels, R., Galagan, J., Cuomo, C., Dobinson, K.F., Ma, L-J. 2011. Comparative genomics yields insights into niche adaptation of plant vascular wilt pathogens. PLoS Pathogens. 7(7): e1002137.
The inheritance of resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante. Reprint Icon - (Peer Reviewed Journal)
Hayes, R.J., Mchale, L.K., Vallad, G.E., Jose-Truco, M., Michelmore, R.W., Klosterman, S.J., Maruthachalam, K., Subbarao, K.V. 2011. The inheritance of resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante. Theoretical and Applied Genetics. DOI 10.1007/s00122-011-1603-y.
One Step Construction of Agrobacterium Recombination-ready-plasmids (OSCAR), an efficient and robust tool for ATMT based gene deletion construction in fungi - (Peer Reviewed Journal)
Zahi, P., Garcia-Pedrajas, M., Andrews, D.L., Klosterman, S.J., Montanez, B., Gold, S.E. 2011. One step construction of agrobacterium recombination-ready-plasmids (OSCAR), an efficient and robust tool for ATMT based gene deletion construction in fungi. Fungal Genetics and Biology. 48:677-684.
One step construction of agrobacterium recombination-ready plasmids (OSCAR), an efficient and robust tool for ATMT based gene deletion construction in fungi. - (Peer Reviewed Journal)
Paz, Z., Garcia-Pedrajas, M.D., Andrews, D.L., Klosterman, S.J., Montanez, B., Gold, S.E. 2011. One step construction of agrobacterium recombination-ready plasmids (OSCAR), an efficient and robust tool for ATMT based gene deletion construction in fungi. Fungal Genetics and Biology. 48:677-684.
Real-time PCR for the quantification of fungi in planta ()
Klosterman, S.J. 2012. Real-time PCR for the quantification of fungi in planta. In: Bolton, M.D., Thomma, B.P.H.J., editors. Plant Fungal Pathogens: Methods and Protocols. Series: Methods in Molecular Biology. Volume 835. New York, NY: Humana Press. p. 121-131.
Iceberg lettuce breeding lines with resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae. - (Peer Reviewed Journal)
Hayes, R.J., Maruthachalam, K., Vallad, G.E., Klosterman, S.J., Simko, I., Luo, Y., Subbarao, K.V. 2011. Iceberg lettuce breeding lines with resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae. HortScience. 46(3):501-504.
Identification of pathogenicity-related genes in the vascular wilt fungus verticillium dahliae by agrobacterium tumefaciens-mediated t-DNA insertional mutagenesis. - (Peer Reviewed Journal)
Maruthachalam, K., Klosterman, S.J., Kang, S., Hayes, R.J., Subbarao, K.V. 2011. Identification of pathogenicity-related genes in the vascular wilt fungus verticillium dahliae by agrobacterium tumefaciens-mediated t-DNA insertional mutagenesis. Molecular Biotechnology. PMID:21424547.
Verticillium comparative genomics yields insights into niche adaptation by plant vascular wilt pathogens Reprint Icon - (Abstract Only)
Klosterman, S.J., Subbarao, K.V., Kang, S., Veronese, P., Gold, S.E., Thomma, B.J., Chen, Z., Henrissat, B., Lee, Y., Park, J., Garcia-Pedrajas, M.D., Barbara, D., Anchieta, A.G., De Jonge, R., Santhanam, P., Maruthachalam, K., Atallah, Z.K., Amyotte, S., Paz, Z., Inderbitzin, P., Hayes, R.J., Heiman, D.I., Young, S., Zeng, Q., Engels, R., Galagan, J., Cuomo, C., Dobinson, K.F., Ma, L. 2011. Verticillium comparative genomics yields insights into niche adaptation by plant vascular wilt pathogens. Plant and Animal Genome Conference. Online.
Molecular variation among isolates of verticillium dahliae and PCR-based differentiation of races. - (Peer Reviewed Journal)
Maruthachalam, K., Atallah, Z.K., Vallad, G.E., Klosterman, S.J., Hayes, R.J., Davis, M.R., Subbarao, K. 2010. Molecular variation among isolates of verticillium dahliae and PCR-based differentiation of races. Phytopathology. 11:1222-30.
Molecular variation among isolates of verticillium dahliae and PCR-based differentiation of races. - (Peer Reviewed Journal)
Maruthachalam, K., Atallah, Z.K., Vallad, G.E., Klosterman, S.J., Hayes, R.J., Davis, R., Subbarao, K.V. 2010. Molecular variation among isolates of verticillium dahliae and PCR-based differentiation of races. Phytopathology. 100(11):1222-30.
A qPCR assay for detection and quantification of Verticillium dahliae in spinach seed. - (Abstract Only)
Rauscher, G.M., Mou, B., Hayes, R.J., Koike, S.T., Maruthachalam, K., Subbarao, K.V., Klosterman, S.J. 2010. A qPCR assay for detection and quantification of Verticillium dahliae in spinach seed.. Phytopathology. 100:S107.
Population analyses of the vascular plant pathogen Verticillium dahliae detect recombination and transcontinental gene flow - (Peer Reviewed Journal)
Atallah, Z.K., Maruthachalam, K.K., Du Toit, L.J., Koike, S.T., R. Michael, D., Klosterman, S.J., Hayes, R.J., Subbarao, K.V. 2010. Population analyses of the vascular plant pathogen, Verticillium dahliae detect recombination and transcontinental gene flow. Fungal Genetics and Biology. 47:416-422.
A soilless Verticillium wilt assay using an early flowering lettuce - (Peer Reviewed Journal)
Klosterman, S.J., Hayes, R.J. 2009. A soilless Verticillium wilt assay using an early flowering lettuce. Plant Disease. 93:691-698,2009.
Characterization of 22 highly polymorphic microsatellite loci in the cosmopolitan fungal plant pathogen Verticillium dahliae. Permanent Genetic Resources added to Molecular Ecology Resources - (Peer Reviewed Journal)
Atallah, Z., Maruthachalam, K., Davis, M.R., Klosterman, S.J., Subbarao, K. 2009. Characterization of 22 highly polymorphic microsatellite loci in the cosmopolitan fungal plant pathogen Verticillium dahliae. Permanent Genetic Resources added to Molecular Ecology Resources. Molecular Ecology Resources. Molecular Ecology Resources 9:1460-1466.
Identification of lettuce genes differentially expressed in a Verticillium dahliae-lettuce interaction by suppression subtractive hybridization - (Abstract Only)
Klosterman, S.J., Anchieta, A.G., Maruthachalam, K., Hayes, R.J., Subbarao, K.V. 2009. Identification of lettuce genes differentially expressed in a Verticillium dahliae-lettuce interaction by suppression subtractive hybridization. Phytopathology Supplement; APSnet (Plant Pathology Online).
Diversity, Pathogenicity And Control of Verticillium Species. - (Peer Reviewed Journal)
Klosterman, S.J., Atallah, Z.K., Vallad, G.E., Subbarao, K.N. 2009. Diversity, Pathogenicity And Control of Verticillium Species.. Annual Review of Phytopathology 2009, 47:39-62.
Verticillium comparative genomics--understanding pathogenicity and diversity. - (Abstract Only)
Klosterman, S.J., Veronese, P., Dobinson, K.F., Subbarao, K.V., Heiman, D.L., Young, S., Kodira, C.D., Alvarado, L., Galagan, J.E., Birren, B., Cuomo, C., Anchieta, A.G., Kang, S., Gold, S.E., Ma, L. 2008. Verticillium comparative genomics--understanding pathogenicity and diversity.. Phytopathology.
Development of rapid pathogenicity assay for Verticillium dahliae using early flowering lettuce. - (Abstract Only)
Klosterman, S.J., Hayes, R.J. Development of rapid pathogenicity assay for Verticillium dahliae using early flowering lettuce.. Phytopathology.
Population biology of Verticillium dahliae isolates from lettuce in the Sallinas Valley of Californis. - (Abstract Only)
Phytopathology 98:S14
Analysis of molecular variability among the isolated of Verticillium dahliae from diverse host species based on florescene-based amplified fragment length polymorphism. - (Abstract Only)
Phytopathology 98:S78
Ubc2, an Ortholog of the Yeast Ste50p Adaptor, Possesses a Basidiomycete-Specific Carboxy terminal Extension Essential for Pathogenicity Independent of Pheromone Response. - (Peer Reviewed Journal)
Klosterman, S.J., Martinez-Espinoza, A.D., Andrews, D.L., Seay, J.R., Gold, S.E. 2008. Ubc2, an Ortholog of the Yeast Ste50p Adaptor, Possesses a Basidiomycete-Specific Carboxy terminal Extension Essential for Pathogenicity Independent of Pheromone Response. Molecular Plant-Microbe Interactions. Vol. 21, No. 1 p. 110-121.
Comparative Verticillium genomics - (Abstract Only)
Klosterman, S.J., Subbarao, K.V., Dobinson, K.F., Veronese, P., Kang, S., Gold, S.E., Ma, L. 2007. Comparative Verticillium genomics. Fungal Genetics Newsletter. 54(Suppl) pg. 75.
Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis - (Peer Reviewed Journal)
Kamper, J., Kahmann, R., Bolker, M., Ma, L., Brefort, T., Saville, B.J., Banuett, F., Kronstad, J.W., Gold, S.E., Muller, O., Perlin, M.H., Wosten, H.A., Devries, R., Ruiz-Herrera, J., Reynaga-Pena, C.G., Snetselaar, K., Mccann, M., Perez-Martin, J., Feldrugge, M., Basse, C.W., Steinberg, G., Ibeas, J., Holloman, W., Guzman, P., Farman, M., Stajich, J.E., Sentandreu, R., Gonzalez-Prietro, J.M., Kennell, J.C., Molina, L., Schirawski, J., Mendoza-Mendoza, A., Greilinger, D., Munch, K., Rossel, N., Scherer, M., Vranes, M., Ladendorf, O., Vincon, V., Fuchs, U., Sandrock, B., Meng, S., Ho, E.C., Cahill, M.J., Boyce, K.J., Klose, J., Klosterman, S.J., Deelstra, H.J., Ortiz-Castellanos, L., Li, W., Sanchez-Alonso, P., Schreier, P.H., Hauser-Hahn, I., Vaupel, M., Koopmann, E., Griedrich, G., Voss, H., Schluter, T., Margolis, J., Platt, D., Swimmer, C., Gnirke, A., Chen, F., Vysotskaia, V., Mannhaupt, G., Guldener, U., Munsterkotter, M., Haase, D., Oesterheld, M., Mewes, H., Maucell, E.W., Decaprio, D., Wade, C.M., Butler, J., Young, S., Jaffe, D.D., Calvo, S., Nusbaum, C., Galagan, J., Birren, B.W. 2006. Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis. Nature. 2006. 444:97-101. DOI:10.1038/nature05248
Genetics of morphogenesis and pathogenic development of Ustilago maydis. ()
Klosterman, S.J., Perlin, M.H., Garcia-Pedrajas, M., Covert, S.F., Gold, S.E. 2007. Genetics of morphogenesis and pathogenic development of Ustilago maydis. Advances in Genetics. Ed. Dunlap, Jay C. Vol. 57. pg. 1-47.