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ARS Home » Pacific West Area » Salinas, California » Crop Improvement and Protection Research » Research » Publications at this Location » Publication #376322

Research Project: Management of Pathogens for Strawberry and Vegetable Production Systems

Location: Crop Improvement and Protection Research

Title: RPA assay detection of spinach downy mildew in leaves

item Klosterman, Steven
item Anchieta, Amy

Submitted to: Meeting Abstract
Publication Type: Other
Publication Acceptance Date: 7/10/2020
Publication Date: 7/10/2020
Citation: Klosterman, S.J., Anchieta, A.G. 2020. RPA assay detection of spinach downy mildew in leaves. Demonstration for the California Leafy Greens Research Program. Available:

Interpretive Summary:

Technical Abstract: Downy mildew disease on spinach, caused by the oomycete pathogen Peronospora effusa, is a major constraint in organic spinach production in California, and elsewhere where spinach is grown. The disease causes yellowing lesions on leaves, which can render them unmarketable. This research describes the application of a recombinase polymerase amplification (RPA) assay for detection of P. effusa in spinach leaves. The RPA assay is an improvement over previous methods to detect P. effusa, as it is more sensitive and faster (result in 20 minutes) than conventional PCR assays, is specific for detection of only P. effusa, and can be conducted in the field for point-of-care use. The RPA assay not only provides a quick detection tool for researchers but can be useful for early detection of P. effusa in organic spinach before the development of symptoms. This knowledge may be useful to a grower to make disease management decisions such as harvesting the crop before it becomes symptomatic.