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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Produce Safety and Microbiology Research » People & Locations » Robert Hnasko

Robert Hnasko
Produce Safety and Microbiology Research
Research Molecular Biologist

Phone: (510) 559-5878
Fax: (510) 559-6429

USDA, ARS, PWA, WRRC-PSM
800 BUCHANAN ST
ALBANY , CA 94710
Projects
Ecology and Detection of Human Pathogens in the Produce Production Continuum
In-House Appropriated (D)
  Accession Number: 430338
Immunodiagnostics to Detect Prions and Other Important Animal Pathogens
In-House Appropriated (D)
  Accession Number: 434529

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Lateral flow immunoassay (LFIA) for the detection of lethal amatoxins from mushrooms Reprint Icon - (Peer Reviewed Journal)
Bever, C.R., Adams, C.A., Hnasko, R.M., Cheng, L.W., Stanker, L.H. 2020. Lateral flow immunoassay (LFIA) for the detection of lethal amatoxins from mushrooms. PLoS One. 15(4):e0231781. https://doi.org/10.1371/journal.pone.0231781.
A Rapid Extraction Method Combined with a Monoclonal Antibody-Based Immunoassay for the Detection of Amatoxins Reprint Icon - (Peer Reviewed Journal)
Bever, C.R., Hnasko, R.M., Cheng, L.W., Stanker, L.H. 2019. A Rapid Extraction Method Combined with a Monoclonal Antibody-Based Immunoassay for the Detection of Amatoxins. Toxins. 11(12):724. https://doi.org/10.3390/toxins11120724.
Rapid detection of staphylococcal enterotoxin-B (SEB) by lateral flow assay Reprint Icon - (Peer Reviewed Journal)
Hnasko, R.M., McGarvey, J.A., Lin, A.V. 2019. Rapid detection of staphylococcal enterotoxin-B (SEB) by lateral flow assay. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 38(5):209-212. https://doi.org/10.1089/mab.2019.0028.
Use of phyllosphere associated lactic acid bacteria as biocontrol agents to reduce salmonella enterica growth on cantaloupe melons - (Peer Reviewed Journal)
Mcgarvey, J.A., Tran, T.D., Hnasko, R.M., Gorski, L.A. 2019. Use of phyllosphere associated lactic acid bacteria as biocontrol agents to reduce salmonella enterica growth on cantaloupe melons. Journal of Food Protection. 82(12)2148-2153.
Development and characterization of monoclonal antibodies to botulinum neurotoxin type E Reprint Icon - (Peer Reviewed Journal)
Bever, C.R., Scotcher, M., Cheng, L.W., Hnasko, R.M., Stanker, L. 2019. Development and characterization of monoclonal antibodies to botulinum neurotoxin type E. Toxins. 11:407. https://doi.org/10.3390/toxins11070407.
Bacterial population dynamics after foliar fertilization of almond leaves Reprint Icon - (Peer Reviewed Journal)
McGarvey, J.A., Han, R., Tran, T., Hnasko, R.M., Brown, P. 2018. Bacterial population dynamics after foliar fertilization of almond leaves. Journal of Applied Microbiology. 126(3):945-953. https://doi.org/10.1111/jam.14169.
Complete genome sequence of Lactococcus lactis subsp. lactis strain 14B4, which inhibits the growth of Salmonella enterica serotype Poona in vitro Reprint Icon - (Peer Reviewed Journal)
Tran, T.D., Huynh, S., Parker, C., Han, R., Hnasko, R.M., Gorski, L.A., McGarvey, J.A. 2018. Complete genome sequence of Lactococcus lactis subsp. lactis strain 14B4, which inhibits the growth of Salmonella enterica serotype Poona in vitro. Microbiology Resource Announcements. 7(19):e01364-18. https://doi.org/10.1128/MRA.01364-18.
Dosage-dependent effects of Monensin on the rumen microbiota of lactating dairy cattle Reprint Icon - (Peer Reviewed Journal)
McGarvey, J.A., Place, S., Palumbo, J.D., Hnasko, R.M., Mitloehner, F. 2018. Dosage-dependent effects of Monensin on the rumen microbiota of lactating dairy cattle. Advances in Dairy Research. 8(7):e00783. https://doi.org/10.1002/mbo3.783.
A new conjugation method used for the development of an immunoassay for amanitin, a deadly mushroom toxin Reprint Icon - (Peer Reviewed Journal)
Bever, C.R., Barnych, B., Hnasko, R.M., Cheng, L.W., Stanker, L.H. 2018. A new conjugation method used for the development of an immunoassay for amanitin, a deadly mushroom toxin. Toxins. 10(7):265. https://doi.org/10.3390/toxins10070265.
A bioassay for the optimization of macrophage conditioned medium (MCM) as culture supplement used to promote hybridoma cell survival and growth Reprint Icon - (Peer Reviewed Journal)
Hnasko, R.M., Lin, A.V., Stanker, L.H., McGarvey, J.A. 2018. A bioassay for the optimization of macrophage conditioned medium (MCM) as culture supplement used to promote hybridoma cell survival and growth. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 37(3):126-133. https://doi.org/10.1089/mab.2018.0008.
Enhanced detection of infectious prions by direct ELISA from the brains of asymptomatic animals using DRM2-118 monoclonal antibody and Gdn-HCl Reprint Icon - (Peer Reviewed Journal)
Hnasko, R.M., Lin, A.V., McGarvey, J.A., Stanker, L.H. 2018. Enhanced detection of infectious prions by direct ELISA from the brains of asymptomatic animals using DRM2-118 monoclonal antibody and Gdn-HCl. Journal of Immunological Methods. 456:38-43. https://doi.org/10.1016/j.jim.2018.02.010.
Identification and verification of hybridoma-derived monoclonal antibody variable region sequences using recombinant DNA technology and mass spectrometry Reprint Icon - (Peer Reviewed Journal)
Babrak, L.M., McGarvey, J.A., Stanker, L.H., Hnasko, R.M. 2017. Identification and verification of hybridoma-derived monoclonal antibody variable region sequences using recombinant DNA technology and mass spectrometry. Molecular Immunology. 90:287-294. https://doi.org/10.1016/j.molimm.2017.08.014.
Rapid microfluidic assay for the detection of botulinum neurotoxin in animal sera - (Peer Reviewed Journal)
Babrak, L.M., Lin, A.V., Stanker, L.H., McGarvey, J.A., Hnasko, R.M. 2016. Rapid microfluidic assay for the detection of botulinum neurotoxin in animal sera. Toxins. 8(1):13.
Monoclonal antibody technologies and rapid detection assays - (Proceedings)
Hnasko, R.M., Stanker, L.H., Mcgarvey, J.A. 2015. Monoclonal antibody technologies and rapid detection assays.September 23-25, 2015 Carlsbad, CA: p. 1-44.
Bacterial populations on the surfaces of organic and conventionally grown almond drupes Reprint Icon - (Peer Reviewed Journal)
Mcgarvey, J.A., Hnasko, R.M., Stanker, L.H., Han, R., Connel, J. 2015. Bacterial populations on the surfaces of organic and conventionally grown almond drupes. Journal of Applied Microbiology. 119:529-538.
ELISA: Methods and Protocols ()
Hnasko, R.M. 2015. ELISA: Methods and Protocols. Methods in Molecular Biology. Volume 1318. New York: Humana Press. 435 p. doi: 10.1007/978-1-4939-2742-5.
The biochemical properties of antibodies and their fragments ()
Hnasko, R.M. 2015. The biochemical properties of antibodies and their fragments. In: Hnasko, R., editor. ELISA: Methods and protocols. Methods in Molecular Biology. 1st edition. New York, NY: Humana Press. p. 1-14. doi: 10.1007/978-1-4939-2742-5.
Hybridoma technology ()
Hnasko, R.M., Stanker, L.H. 2015. Hybridoma technology. In: Hnasko, R., editor. ELISA: Methods and protocols. Methods in Molecular Biology. 1st edition. New York, NY: Humana Press. p. 15-28. doi: 10.1007/978-1-4939-2742-5.
Bioconjugation of antibodies to horseradish peroxidase (hrp) ()
Hnasko, R.M. 2015. Bioconjugation of antibodies to horseradish peroxidase (hrp).In: Hnasko, R., editor. ELISA: Methods and protocols. Methods in Molecular Biology. 1st edition. New York, NY: Humana Press. p. 43-50. doi: 10.1007/978-1-4939-2742-5.
Affinity purification of antibodies ()
Hnasko, R.M., McGarvey, J.A. 2015. Affinity purification of antibodies. In: Hnasko, R., editor. ELISA Methods and Protocols. New York, NY: Humana Press. p. 29-41. doi: 10.1007/978-1-4939-2742-5.
The western blot ()
Hnasko, R.M., Hnasko, T.S. 2015. The western blot. In: Hnasko, Robert, editor. ELISA: Methods and protocols. Methods in Molecular Biology. 1st edition. New York, NY:Humana Press. p. 87-96. doi: 10.1007/978-1-4939-2742-5.
Rapid detection of E. coli produced shiga-like toxins by lateral flow immunoassay in multiple food matrices - (Peer Reviewed Journal)
Ching, K.H., He, X., Stanker, L.H., Lin, A.V., Hnasko, R.M. 2015. Rapid detection of E. coli produced shiga-like toxins by lateral flow immunoassay in multiple food matrices. Toxins. Toxins 7:1163-1173.
Detection of shiga toxins by lateral flow assay - (Peer Reviewed Journal)
Ching, K.H., He, X., Stanker, L.H., Lin, A.V., Mcgarvey, J.A., Hnasko, R.M. 2015. Detection of shiga toxins by lateral flow assay. Toxins. 7:1163-1173. doi: 10.3390/Toxins7041163.
Prion extraction methods: comparison of bead beating, ultrasonic disruption and repeated freeze-thaw methodologies for the recovery of functional renilla-prion fusion protein from bacteria ()
Hnasko, R.M., Lin, A.V., Stanker, L.H., Bala, K., McGarvey, J.A. 2016. Prion extraction methods: comparison of bead beating, ultrasonic disruption and repeated freeze-thaw methodologies for the recovery of functional renilla-prion fusion protein from bacteria. In: Micic, M., editor. Sample Preparation Techniques for Soil, Plant, and Animal Samples. New York, NY: Humana Press. p. 389-399.
A double-sandwich ELISA for identification of monoclonal antibodies suitable for sandwich immunoassays ()
Stanker, L.H., Hnasko, R.M. 2015. A double-sandwich ELISA for identification of monoclonal antibodies suitable for sandwich immunoassays. Methods in Molecular Biology. Springer Science and Business Media, New York. 216 pp. 2015. (Book Chapter.
The generation of monoclonal antibodies and their use in rapid diagnostic tests - (Proceedings)
Hnasko, R.M., Stanker, L.H., Mcgarvey, J.A., Lin, A.V. 2014. The generation of monoclonal antibodies and their use in rapid diagnostic tests. Proceedings: Microfluidics Regenerated: New Approaches, Applications and Enabling Technologies. Meeting Abstract. 7(1-16).
Bacterial population structure and dynamics during the development of almond drupes Reprint Icon - (Peer Reviewed Journal)
McGarvey, J.A., Connell, J., Stanker, L.H., Hnasko, R.M. 2014. Bacterial population structure and dynamics during the development of almond drupes. Journal of Applied Microbiology. 116:1542-1552.
A monoclonal antibody based capture ELISA for botulinum neurotoxin serotype B: toxin detection in food Reprint Icon - (Peer Reviewed Journal)
Stanker, L.H., Scotcher, M.C., Cheng, L.W., Ching, K., Mcgarvey, J.A., Hodge, D., Hnasko, R.M. 2013. A monoclonal antibody based capture ELISA for botulinum neurotoxin serotype B: toxin detection in food. Toxins. 5(11):2212-2226.
A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by escherichia coli in human and environmental samples - (Peer Reviewed Journal)
He, X., Patfield, S.A., Hnasko, R.M., Rasooly, R., Mandrell, R.E. 2013. A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by escherichia coli in human and environmental samples. PLoS One. 8(10):e76368.
Detection of bacterial toxins by lateral flow immunoassay - (Proceedings)
Ching, K.H., Stanker, L.H., He, X., Hnasko, R.M. 2013. Detection of bacterial toxins by lateral flow immunoassay. p. 1-28. Irvine, CA; Biodot.
Bacterial population dynamics during the ensiling of Medicago sativa (alfalfa) and subsequent exposure to air Reprint Icon - (Peer Reviewed Journal)
McGarvey, J.A., Franco, R., Palumbo, J.D., Hnasko, R.M., Stanker, L.H., Mitloehner, F. 2013. Bacterial population dynamics during the ensiling of Medicago sativa (alfalfa) and subsequent exposure to air. Journal of Applied Microbiology. 114:1661-1670.
Development and characterization of six monoclonal antibodies to hemagglutinin-70 (HA70) of Clostridium botulinum and their application in a sandwich ELISA - (Peer Reviewed Journal)
Scotcher, M.C., Cheng, L.W., Ching, K.H., Mcgarvey, J.A., Hnasko, R.M., Stanker, L.H. 2013. Development and characterization of six monoclonal antibodies to hemagglutinin-70 (HA70) of Clostridium botulinum and their application in a sandwich ELISA. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 32(1):6-15. doi: 10.1089/mab.2012.0071.
Rapid multiplex immunoassay to distinguish botulinum neurotoxin serotypes on a single lateral flow device(Abstract) - (Abstract Only)
Novel epitopes identified by Anti-PrP monoclonal antibodies produced following immunization of Prnp0/0 Balb/cJ mice with purified scrapie prions - (Peer Reviewed Journal)
Stanker, L.H., Scotcher, M.C., Lin, A.V., Mcgarvey, J.A., Prusiner, S., Hnasko, R.M. 2012. Novel epitopes identified by Anti-PrP monoclonal antibodies produced following immunization of Prnp0/0 Balb/cJ mice with purified scrapie prions. Hybridoma. 31(5):312-324. doi:10.1089/hyb.2012.0022.
Replacement of animals in assays for detection of toxins in food(Abstract) - (Abstract Only)
Rapid and selective detection of botulinum neurotoxin serotypes-A and –B with a single immunochromatographic test strip Reprint Icon - (Peer Reviewed Journal)
Ching, K.H., Lin, A.V., Mcgarvey, J.A., Stanker, L.H., Hnasko, R.M. 2012. Rapid and selective detection of botulinum neurotoxin serotypes-A and –B with a single immunochromatographic test strip. Journal of Immunological Methods. 380:23-29.
A rapid method to improve protein detection by indirect ELISA - (Peer Reviewed Journal)
Hnasko, R.M., Lin, A.V., Mcgarvey, J.A., Stanker, L.H. 2011. A rapid method to improve protein detection by indirect ELISA. Biochemical and Biophysical Research Communications. 410(4):726-731. doi: 10.1016/j.bbrc.2011.06.005.
Generation of Antisera to Purified Prions in Lipid Rafts - (Peer Reviewed Journal)
Hnasko, R.M., Serban, A.V., Carlson, G.A., Prusiner, S.B., Stanker, L.H. 2010. Generation of Antisera to Purified Prions in Lipid Rafts. Prion. 4:294-104.
Conformation-dependent high-affinity mAbs to prion proteins - (Peer Reviewed Journal)
Stanker, L.H., Serban, A.V., Safar, J., Hnasko, R.M., Prusiner, S.B. 2010. Conformation-dependent high-affinity mAbs to prion proteins. Journal of Immunology. 185:729-737.
Conformation-Dependent High-Affinity Monoclonal Antibodies to Prion Proteins - (Peer Reviewed Journal)
Stanker, L.H., Serban, A.V., Cleveland, E., Hnasko, R.M., Azucena, L., Safar, J., Dearmond, S.J., Prusiner, S.B. 2010. Isolation and characterization of new anti-PrP monoclonal antibodies. Journal of Immunology. 185: 729-737
Generation of Monoclonal Antibodies to Purified Prions in Lipid Rafts - (Abstract Only)
Inoculation of Scrapie with the Self-Assembling RADA-Peptide Disrupts Prion Accumulation and Extends Hamster Survival - (Peer Reviewed Journal)
Hnasko, R.M., Bruederle, C.E. 2009. Inoculation of Scrapie with the Self-Assembling RADA-Peptide Disrupts Prion Accumulation and Extends Hamster Survival. PLoS ONE. 4(2): http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004440
Prion infected Meat-and-Bone Meal is still infectious after biodiesel production - (Peer Reviewed Journal)
Bruederle, C.E., Hnasko, R.M., Kraemer, T., Garcia, R.A., Haas, M.J., Marmer, W.N., Carter, J.M. 2008. Prion infected Meat-and-Bone Meal is still Infectious After Biodiesel Production. PLoS One 3(8): e2969. doi:10.1371/journal.pone.0002969.
Sensitive, Preclinical Detection of Prions in Brain by nanospray liquid chromatography/tandem mass spectrometry - (Peer Reviewed Journal)
Onisko, B.C., Silva, C.J., Dynin, I.A., Erickson, M.L., Vensel, W.H., Hnasko, R.M., Requena, J.R., Carter, J.M. 2007. Sensitive, Preclinical Detection of Prions in Brain by nanospray liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry. 21(24):4023-4026.
Genetic disruption of dopamine production results in pituitary adenomas and severe prolactinemia - (Peer Reviewed Journal)
Hnasko, T.S., Hnasko, R.M., Sotak, B.N., Kapur, R.P. 2007. Genetic disruption of dopamine production results in pituitary adenomas and severe prolactinemia. Neuroendocrinology Journal. 86(1): 48-57
PV-1 LABELS TRANS-CELLULAR OPENINGS IN MOUSE ENDOTHELIAL CELLS AND IS NEGATIVELY REGULATED BY VEGF - (Peer Reviewed Journal)
Hnasko, R.M., Carter, J.M., Medina, F., Lisanti, M. 2006. Pv-1 labels trans-cellular openings in mouse endothelial cells and is negatively regulated by vegf. Cell Cycle. 5(17):2021-28.
PV-1 IS NEGATIVELY REGULATED BY VEGF IN THE LUNG OF CAV-1, BUT NOT CAV-2, NULL MICE - (Peer Reviewed Journal)
Hnasko, R.M., Philippe, F.G., Ben-Jonathan, N., Lisanti, M.P. 2006. Pv-1 is negatively regulated by vegf in the lung of cav-1, but not cav-2, null mice. Cell Cycle 5(17):2012-20.
Loss of Nkx2.8 deregulates progenitor cells in the large airways and leads to dysplasia - (Peer Reviewed Journal)
Tian, J., Radma, M., Hnasko, R.M., Locker, J. 2006. Loss of Nkx2.8 deregulates progenitor cells in the large airways and leads to dysplasia. Cancer Research. 1:66(21):10399-407.
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