Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: 6/6/2014
Publication Date: 6/19/2014
Citation: Hnasko, R.M., Stanker, L.H., Mcgarvey, J.A., Lin, A.V. 2014. The generation of monoclonal antibodies and their use in rapid diagnostic tests. Proceedings: Microfluidics Regenerated: New Approaches, Applications and Enabling Technologies. Meeting Abstract. 7(1-16).
Technical Abstract: Antibodies are the most important component of an immunoassay. In these proceedings we outline novel methods used to generate and select monoclonal antibodies that meet performance criteria for use in rapid lateral flow and microfluidic immunoassay tests for the detection of agricultural pathogens and their byproducts. Novel data is presented on the chemistry and methods used in antibody immobilization to various thermoplastics that improve assay sensitivity. Additional data is presented on the use of novel reagents to effectively block non-specific protein interactions with antibody binding sites and surfaces to limit unwanted sample interference, improve signal generation and enhance antigen detection sensitivity.