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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Produce Safety and Microbiology Research » Research » Publications at this Location » Publication #305615

Research Project: Immunodiagnostics to Detect Prions and Other Important Animal Pathogens

Location: Produce Safety and Microbiology Research

Title: ELISA: Methods and Protocols

Author
item Hnasko, Robert

Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter
Publication Acceptance Date: 4/4/2015
Publication Date: 7/14/2015
Citation: Hnasko, R.M. 2015. ELISA: Methods and Protocols. Methods in Molecular Biology. Volume 1318. New York: Humana Press. 435 p. doi: 10.1007/978-1-4939-2742-5.

Interpretive Summary: This book provides the reader with the necessary knowledge on how to use a variety of immunoassay strategies, reporters and detection systems to facilitate their efforts to gain answers to their own scientific questions. It is the goal of this book to provide the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.

Technical Abstract: The antibody is central to the performance of an ELISA providing the basis of analyte selection and detection. It is the interaction of antibody with analyte under defined conditions that dictates the outcome of the ELISA and deviations in those conditions will impact assay performance. The aim of this manual is to provide the reader an overview of ELISA techniques and their potential application along with the technical aptitude necessary to design, run, and evaluate these immunoassays for the detection and quantitation of a target analytes in solution.