Location: Produce Safety and Microbiology ResearchTitle: Rapid detection of staphylococcal enterotoxin-B by lateral flow assay
Submitted to: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/28/2019
Publication Date: 10/10/2019
Citation: Hnasko, R.M., McGarvey, J.A., Lin, A.V. 2019. Rapid detection of staphylococcal enterotoxin-B by lateral flow assay. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 38(5):209-212. https://doi.org/10.1089/mab.2019.0028.
Interpretive Summary: Enterotoxins are produced by a common bacterium that can contaminate foods and when ingested these toxins may cause food poisoning. One of these toxins termed staphylococcus enterotoxin-B (SEB) is considered most dangerous as it is highly toxic and can withstand high temperatures associated with cooking. We have developed a rapid, portable and sensitive test strip that can detect SEB in an inexpensive and easy-to-use format to improve food safety.
Technical Abstract: The pathogenic bacterium Staphylococcus aureus produces enterotoxins (SE) that cause foodborne illness. We have generated 24 monoclonal antibodies (MAbs) against staphylococcal enterotoxin-B (SEB) which is considered the most prevalent and dangerous. These antibodies bind the SEB protein and function in a variety of immunoassay formats suitable for detection. We identified a pair of anti-SEB MAbs and developed those into a rapid and portable lateral flow assay. These SEB test strips provide rapid and portable SEB detection with a sensitivity of 5 ng/mL using visual colorimetric reporter.