Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter
Publication Acceptance Date: 4/4/2015
Publication Date: 7/14/2015
Citation: Hnasko, R.M., Stanker, L.H. 2015. Hybridoma technology. In: Hnasko, R., editor. ELISA: Methods and protocols. Methods in Molecular Biology. 1st edition. New York, NY: Humana Press. p. 15-28. doi: 10.1007/978-1-4939-2742-5.
Interpretive Summary: This manuscript details how one generates and characterizes antibodies against specific target antigens.
Technical Abstract: The generation of hybridoma cell lines by the fusion of splenocytes from immunized mice with immortal myeloma cells is a well established method for the production of monoclonal antibodies. Although other methods have emerged as an effective alternative for the generation of monoclonal antibodies the use of hybridoma technology remain a viable technique that is accessible to wide number of laboratories that perform basic cell biological research. Hybridoma technology represents a relatively simple procedure at minimal cost for the continuous production of native whole immunoglobulin. This chapter will describe the materials and methodologies needed for the successful generation of monoclonal antibody (mAb) producing hybridoma cell lines against target antigens.