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United States Department of Agriculture

Agricultural Research Service

Related Topics

Rong Wang
Meats Safety & Quality Research
Microbiology

Phone: (402) 762-4228
Fax: (402) 762-4149

P.O. BOX 166
CLAY CENTER , NE 689330166

Projects
Genomic and Metagenomic Differences in Foodborne Pathogens and Determination of Ecological Niches and Reservoirs
Appropriated (D)
  Accession Number: 430418
Mitigation Approaches for Foodborne Pathogens in Cattle and Swine for Use During Production and Processing
Appropriated (D)
  Accession Number: 430703

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Characterization of enterohemorrhagic E. coli on veal hides and carcasses -
Escherichia coli O157:H7 strains isolated from “High Event Period” beef contamination have strong biofilm forming ability and low sanitizer susceptibility which are associated with high pO157 plasmid copy number -
Complete and closed genome sequences of 10 Salmonella enterica subsp. enterica serovar Anatum isolated from human and bovine sources Reprint Icon -
Nguyen, S.V., Harhay, D.M., Bono, J.L., Smith, T.P.L., Fields, P.I., Dinsmore, B.A., Santovenia, M., Kelley, C.M., Wang, R., Bosilevac, J.M., Harhay, G.P. 2016. Complete and closed genome sequences of 10 Salmonella enterica subsp. enterica serovar Anatum isolated from human and bovine sources. Genome Announcements. Vol. 4(3): e00447-16. doi: 10.1128/genomeA.00447-16.
Contamination revealed by indicator microorganism levels during veal processing Reprint Icon -
Bosilevac, J.M., Wang, R., Luedtke, B.E., Wheeler, T.L., Koohmaraie, M. 2016. Contamination revealed by indicator microorganism levels during veal processing. Journal of Food Protection. 79(8):1341-1347. doi:10.4315/0362-028X.JFP-15-572
Evaluation of Salmonella biofilm cell transfer from contact surfaces to beef products -
Wang, R., King, D.A., Harhay, D.M., Arthur, T.M. 2016. Evaluation of Salmonella biofilm cell transfer from contact surfaces to beef products. [Abstract] International Association for Food Protection. Supp. A(79):102. P1-64.
Development and application of a green fluorescent protein (GFP) expressing E. coli O103 surrogate for tracking contamination through grinding and identifying persistent points of contamination -
Bosilevac, J.M., Luedtke, B.E., Wang, R., Ogunrinola, Y. 2016. Development and application of a green fluorescent protein (GFP) expressing E. coli O103 surrogate for tracking contamination through grinding and identifying persistent points of contamination.[abstract] Beef Industry Safety Summit, March 1-3, 2016, Austin, Texas. Available: http://www.bifsco.org/2016researchsessionabstracts.aspx.
Mobilome differences between Salmonella enterica serovars Anatum and Typhimurium isolated from cattle and humans and potential impact on virulence -
Nguyen, S.V., Bono, J.L., Smith, T.P.L., Kelley, C.M., Wang, R., Harhay, G.P., Harhay, D.M. 2016. Mobilome differences between Salmonella enterica serovars Anatum and Typhimurium isolated from cattle and humans and potential impact on virulence. [Abstract] In proceedings: 60th Wind River Conference on Procaryotic Biology, 2016 June 1-5. Estes Park, Colorado. p. 67.
Diagnostic accuracy of rectoanal mucosal swab of feedlot cattle for detection and enumeration of Salmonella enterica Reprint Icon -
Agga, G.E., Arthur, T.M., Schmidt, J.W., Wang, R., Harhay, D.M. 2016. Diagnostic accuracy of rectoanal mucosal swab of feedlot cattle for detection and enumeration of Salmonella enterica. Journal of Food Protection. 79(4):531-537. doi:10.4315/0362-028X.JFP-15-409.
Complete closed genome sequences of Salmonella enterica subsp. enterica serotypes Anatum, Montevideo, Typhimurium and Newport, isolated from beef, cattle, and humans Reprint Icon -
Harhay, D.M., Bono, J.L., Smith, T.P., Fields, P.I., Dinsmore, B.A., Santovenia, M., Kelley, C.M., Wang, R., Harhay, G.P. 2016. Complete closed genome sequences of Salmonella enterica subsp. enterica serotypes Anatum, Montevideo, Typhimurium and Newport, isolated from beef, cattle, and humans. Genome Announcements. 4(1):e01683-15. doi:10.1128/genomeA.01683-15.
Sequence of colonization determines the composition of mixed biofilms by Escherichia coli O157:H7 and O111:H8 strains Reprint Icon -
Wang, R., Kalchayanand, N., Bono, J.L. 2015. Sequence of colonization determines the composition of mixed biofilms by Escherichia coli O157:H7 and O111:H8 strains. Journal of Food Protection. 78(8):1554-1559. DOI: 10.4315/0362-028X.JFP-15-009.
Comparative analysis of twenty-four complete aenome aequences of Salmonella enterica Serotypes Anatum, Montevideo, Typhimurium and Newport isolated from ground beef or asymptomatic cattle on farm or at harvest -
Harhay, D.M., Bono, J.L., Smith, T.P., Kalbfleisch, T., Kelley, C., Wang, R., Harhay, G.P. 2015. Comparative analysis of twenty-four complete aenome aequences of Salmonella enterica Serotypes Anatum, Montevideo, Typhimurium and Newport isolated from ground beef or asymptomatic cattle on farm or at harvest [abstract]. American Society for Microbiology 115th General Meeting. Poster No. 397, Session 029.
Characterization of E. coli O157:H7 strains isolated from “High Event Period” beef trim contamination -
Wang, R., Luedtke, B.E., Kalchayanand, N., Schmidt, J.W., Bosilevac, J.M., Arthur, T.M. 2015. Characterization of E. coli O157:H7 strains isolated from “High Event Period” beef trim contamination. [Abstract] International Association for Food Protection Proceedings. Volume 78 (Supplement A): page 64. (T10-211).
Genomic comparison of Salmonella enterica serotypes commonly associated with cattle and beef – Analysis of variation in virulence potential -
Harhay, D.M., Bono, J.L., Wang, R., Smith, T.P., Harhay, G.P., Kalbfleisch, T., Wheeler, T.L. 2015. Genomic comparison of Salmonella enterica serotypes commonly associated with cattle and beef – Analysis of variation in virulence potential. [Abstract]. Beef Industry Safety Summit. http://www.bifsco.org/2015researchsessionabstracts.aspx.
Efficacy of antimicrobial compounds on surface decontamination of seven shiga toxin-producing Escherichia coli (STEC) and Salmonella inoculated onto fresh beef Reprint Icon -
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Schmidt, J.W., Wang, R., Shackelford, S.D., Wheeler, T.L. 2015. Efficacy of antimicrobial compounds on surface decontamination of seven shiga toxin-producing Escherichia coli and Salmonella inoculated onto fresh beef. Journal of Food Protection. 78(3):503-510.
Occurrence of antimicrobial-resistant Escherichia coli and Salmonella enterica in the beef cattle production and processing continuum Reprint Icon -
Schmidt, J.W., Agga, G.E., Bosilevac, J.M., Harhay, D.M., Shackelford, S.D., Wang, R., Wheeler, T.L., Arthur, T.M. 2015. Occurrence of antimicrobial-resistant Escherichia coli and Salmonella enterica in the beef cattle production and processing continuum. Applied and Environmental Microbiology. 81(2):713-725.
Biofilm formation and sanitizer resistance of Escherichia coli 0157:H7 strains isolated from "High Event Period" meat contamination Reprint Icon -
Wang, R., Kalchayanand, N., King, D.A., Luedtke, B.E., Bosilevac, J.M., Arthur, T.M. 2014. Biofilm formation and sanitizer resistance of Escherichia coli 0157:H7 strains isolated from "High Event Period" meat contamination. Journal of Food Protection. 77(11):1982-1987.
Effect of direct-fed microbial supplementation on the presence of Salmonella enterica in bovine peripheral lymph nodes -
Harhay, D.M., Loneragan, G.H., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Wheeler, T.L., Kolath, B., Theunick, D., Riemann, M., Schaefer, D., Brown, T. 2014. Effect of direct-fed microbial supplementation on the presence of Salmonella enterica in bovine peripheral lymph nodes. [Abstract]. International Association for Food Protection. Supp. A(77):211-212. P3-65.
Effect of residual sanitizers on Salmonella enterica biofilm formation -
Wang, R., Harhay, D.M. 2014. Effect of residual sanitizers on Salmonella enterica biofilm formation. [Abstract] International Association for Food Protection. Supp. A(77):185. P2-163.
Examination of predictors of Salmonella enterica contamination in cattle feedlot environments -
Harhay, D.M., Arthur, T.M., Schmidt, J.W., Wang, R., Kalchayanand, N., Bosilevac, J.M., Edrington, T.S., Loneragan, G., Wheeler, T.L. 2014. Examination of predictors of Salmonella enterica contamination in cattle feedlot environments. [Abstract] Beef Industray Safety Summit. Available: www.bifsco.org/attendees.aspx.
Minimal heat treatments reduce Shiga toxin-producing Escherichia coli (STEC)and Salmonella on the surface of fresh beef -
Kalchayanand, N., Wang, R., Wheeler, T.L. 2014. Minimal heat treatments reduce Shiga toxin-producing Escherichia coli (STEC)and Salmonella on the surface of fresh beef.[Abstract] Annual Meeting of the Institute of Food Technologists. Available:http://www.ift.org/Meetings-and-Events/Past-Meeting-Resources/Technical-Abstract-Search.
Effects of in-plant interventions on reduction of enterohemorrhagic Escherichia coli and background indicator microorganisms on veal calf hides Reprint Icon -
Wang, R., Koohmaraie, M., Wheeler, T.L., Bosilevac, J.M. 2014. Effects of in-plant interventions on reduction of enterohemorrhagic Escherichia coli and background indicator microorganisms on veal calf hides. Journal of Food Protection. 77(5):745-751.
Immersion in antimicrobial solutions reduces Salmonella enterica and Shiga toxin-producing Escherichia coli on beef cheek meat Reprint Icon -
Schmidt, J.W., Bosilevac, J.M., Kalchayanand, N., Wang, R., Wheeler, T.L., Koohmaraie, M. 2014. Immersion in antimicrobial solutions reduces Salmonella enterica and Shiga toxin-producing Escherichia coli on beef cheek meat. Journal of Food Protection. 77(4):538-548.
Impact of sampling area and location on measurement of indicator organisms during beef carcass interventions Reprint Icon -
Wang, R., King, D.A., Koohmaraie, M., Bosilevac, J.M. 2013. Impact of sampling area and location on measurement of indicator organisms during beef carcass interventions. Journal of Food Protection. 76(12):2069-2073.
Mixed biofilm formation by Shiga toxin-producing Escherichia coli and Salmonella enterica serovar typhimurium enhanced bacterial resistance to sanitization due to extracellular polymeric substances Reprint Icon -
Wang, R., Kalchayanand, N., Schmidt, J.W., Harhay, D.M. 2013. Mixed biofilm formation by Shiga toxin-producing Escherichia coli and Salmonella enterica serovar typhimurium enhanced bacterial resistance to sanitization due to extracellular polymeric substances. Journal of Food Protection. 76(9):1513-1522.
Biofilm formation and sanitizer resistance contributes to “High Event” meat contamination by E. coli O157:H7 -
Wang, R., Kalchayanand, N., Bosilevac, J.M., Arthur, T.M. 2013. Biofilm formation and sanitizer resistance contributes to “High Event” meat contamination by E. coli O157:H7. International Association for Food Protection Proceedings. [abstract] P1-48 Page No.48.
Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest Reprint Icon -
Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Edrington, T.S., Brichta-Harhay, D.M. 2013. Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest. Foodborne Pathogens and Disease. 10:368-374.
Salmonella in lymph nodes of cull and fed cattle at harvest -
Webb, H.E., Harhay, D.M., Gragg, S.E., Brashears, M.M., Nightingale, K.K., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Lonergan, G.H. 2013. Salmonella in lymph nodes of cull and fed cattle at harvest. IN: Proceedings of The Beef Industry Summit 2013, March 13-15, 2013, Dallas Texas, 2013 FLASHDRIVE.
Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest Reprint Icon -
Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Edrington, T.S., Harhay, D.M. 2013. Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest. Foodborne Pathogens and Disease. 10(4):368-374.
The physiologic state of Escherichia coli O157:H7 does not affect its detection in two commercial real-time PCR-based tests Reprint Icon -
Wang, R., Schmidt, J.W., Arthur, T.M., Bosilevac, J.M. 2013. The physiologic state of Escherichia coli O157:H7 does not affect its detection in two commercial real-time PCR-based tests. Food Microbiology. 33: 205-212.
Dual-serotype biofilm formation by Shiga toxin-producing Escherichia coli 0157:H7 and 026:H11 strains -
SWang, R., Kalchayanand, N., Schmidt, J.W., Bosilevac, J.M., Bono, J.L. 2012. Dual-serotype biofilm formation by Shiga toxin-producing Escherichia coli 0157:H7 and 026:H11 strains. Applied and Environmental Microbiology. 78(17):6341-6344.
Biofilm formation by Shiga toxin–producing Escherichia coli O157:H7 and non-O157 strains and their tolerance to sanitizers commonly used in the food processing environment Reprint Icon -
Wang, R., Bono, J.L., Kalchayanand, N., Shackelford, S.D., Harhay, D.M. 2012. Biofilm formation by Shiga toxin–producing Escherichia coli O157:H7 and non-O157 strains and their tolerance to sanitizers commonly used in the food processing environment. Journal of Food Protection. 75(8):1418-1428.
Evaluation of commonly used antimicrobial interventions for fresh beef inoculated with Shiga toxin-producing Escherichia coli serotypes O26, O45, O103, O111, O121, O145, and O157:H7 -
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Schmidt, J.W., Wang, R., Shackelford, S.D., Wheeler, T.L. 2012. Evaluation of commonly used antimicrobial interventions for fresh beef inoculated with Shiga toxin-producing Escherichia coli serotypes O26, O45, O103, O111, O121, O145, and O157:H7. Journal of Food Protection. 75(7):1207-1212.
Biofilm formation by foodborne pathogens and their resistance to sanitization -
Wang, R., Bono, J.L., Kalchayanand, N., Shackelford, S.D., Harhay, D.M. 2012. Biofilm formation by foodborne pathogens and their resistance to sanitization. [Abstract]. Americian Society for Microbiology, 112th General Meeting. Poster No 2154, session 170.
Efficacy of hypobromous acid as a hide-on carcass antimicrobial intervention Reprint Icon -
Schmidt, J.W., Wang, R., Kalchayanand, N., Wheeler, T.L., Koohmaraie, M. 2012. Efficacy of hypobromous acid as a hide-on carcass antimicrobial intervention. Journal of Food Protection. 75 (5): 955-958.
Microbiological analysis of bovine lymph nodes for the detection of Salmonella enterica -
Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Shackelford, S.D., Loneragan, G.H., Wheeler, T.L. 2012. Microbiological analysis of bovine lymph nodes for the detection of Salmonella enterica. Journal of Food Protection. 75(5):854-858.
Salmonella in lymph nodes of cattle presented for harvest -
Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Brichta-Harhay, D.M. 2011. Salmonella in lymph nodes of cattle presented for harvest [abstract]. Proceeding of the 2011 Conference of Research Workers in Animal Diseases, December 4-6, 2011, Chicago, Illinois. p. 149.
Salmonella in lymph nodes of cattle presented for harvest -
Gragg, S.E., Loneragan, G.H., Brichta-Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Brashears, M.M. 2011. Salmonella in lymph nodes of cattle presented for harvest [abstract]. International Association for Food Protection. p. 19. Abstract No. P1-62.
Towards an understanding of how Salmonella potentially evades beef carcass processing interventions -
Brichta-Harhay, D.M., Gragg, S.E., Smith, T.P.L., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Shackelford, S.D., Wheeler, T.L., Brashears, M.M., Brooks, J.C., Brown, T.R., Harhay, G.P., Loneragan, G.H. 2011. Towards an understanding of how Salmonella potentially evades beef carcass processing interventions [abstract]. 2011 Beef Industry Safety Summit. Abstract No. 2.
Last Modified: 5/2/2016
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