Page Banner

United States Department of Agriculture

Agricultural Research Service

Related Topics

Deven See

Research Geneticist


 /ARSUserFiles/40746/Lab Staff for Genotyping.jpg

Research Specialty and Interest:

Genotyping of Germplasm for the Western US

Genotyping of Germplasm for the Western US

Development of molecular marker resources for high-throughput genotyping to advance genomic, genetic and breeding research in wheat and barley

Characterization of stress resistance genes and mechanisms

 Western Regional Small Grains Genotyping Lab Page

Research at the WRSGGL involves pre-breeding and genomic studies to identify and develop germplasm with improved adaptation, abiotic and biotic resistances. Genomic research includes Transcriptome profiling, marker development and genetic mapping. Pre-breeding efforts are focused on understanding adaptation traits, and characterizing new sources of resistance to rust pathogens through development of molecular maps to identify and clone genes of interest and to develop breeder- friendly markers for introgression.

Services provided by the WRSGGL include genotyping services for marker assisted selection (MAS) efforts for wheat and barley cultivar development in the Western region. Currently, the genotyping lab is able to provide services including genotyping of early and advance breeding lines; marker assisted selection of backcross populations; marker data for mapping populations; and DNA fingerprints for varietal differentiation. The marker types routinely run include SSR and SNP. The implementation of new genomics' technologies and computational capacity are high priorities to ensure that the wheat and barley scientists serviced by the WRSGGL are at the front edge of modern molecular breeding. Molecular marker genotyping will increasingly provide more efficient information to wheat and barley breeders to increase the efficiency of the breeding effort.

 

Publications

(Clicking on the reprint iconReprint Icon will take you to the publication reprint.)
Registration of “Pritchett” soft white winter club wheat-(Peer Reviewed Journal)
Molecular mapping of stripe rust resistance gene Yr76 in winter club wheat cultivar Tyee-(Peer Reviewed Journal)
Xiang, C., Feng, J., Wang, M., Chen, X., See, D.R., Wan, A., Wang, T. 2016. Molecular mapping of stripe rust resistance gene Yr76 in winter club wheat cultivar Tyee. Phytopathology. doi: 10.1094/PHYTO-01-16-0045-FI.
Secreted protein gene derived-single nucleotide polymorphisms (SP-SNPs) reveal population diversity and differentiation of Puccinia striiformis f. sp. tritici in the United States-(Peer Reviewed Journal)
Xia, C.J., Wang, M.N., Wan, A.M., Jiwan, D.A., See, D.R., Chen, X. 2016. Secreted protein gene derived-single nucleotide polymorphisms (SP-SNPs) reveal population diversity and differentiation of Puccinia striiformis f. sp. tritici in the United States. Fungal Biology. 120(5):729-744.
Association analysis of SP-SNPs and Avr genes in Puccinia striiformis f. sp. tritici, the wheat stripe rust pathogen-(Peer Reviewed Journal)
Xia, C., Wang, M., Wan, A., Jiwan, D.A., See, D.R., Chen, X. 2016. Association analysis of SP-SNPs and Avr genes in Puccinia striiformis f. sp. tritici, the wheat stripe rust pathogen. American Journal of Plant Sciences. 7:126-137.
Identification of somatic hybridization between Puccinia striiformis f. sp. tritici and P. striiformis f. sp. hordei through virulence and molecular characterization of isolates from wheat, barley and grasses-(Peer Reviewed Journal)
Cheng, P., Chen, X., See, D.R. 2016. Identification of somatic hybridization between Puccinia striiformis f. sp. tritici and P. striiformis f. sp. hordei through virulence and molecular characterization of isolates from wheat, barley and grasses. Phytopathology. 106(4):362-371.
Genetic diversity among wheat accessions from the USDA National Small Grains Collection-(Peer Reviewed Journal)
Bonman, J.M., Babiker, E.M., Cuesta-Marcos, A., Esvelt Klos, K.L., Brown Guedira, G.L., Chao, S., See, D.R., Chen, J., Akhunov, E., Zhang, J., Bockelman, H.E., Gordon, T.C. 2015. Genetic diversity among wheat accessions from the USDA National Small Grains Collection. Crop Science. 55(3):1243-1253.
Molecular mapping of YrSP and its relationship with other genes for stripe rust resistance in wheat chromosome 2BL-(Peer Reviewed Journal)
Feng, J., Wang, M., Chen, X., See, D.R., Zheng, Y., Chao, S., Wan, A. 2015. Molecular mapping of YrSP and its relationship with other genes for stripe rust resistance in wheat chromosome 2BL. Phytopathology. 105(9):1206-1213.
Mapping a large number of QTL for durable resistance to stripe rust in winter wheat Druchamp using SSR and SNP markers-(Peer Reviewed Journal)
Hou, L., Chen, X., Wang, M., See, D.R., Chao, S., Bulli, P., Jing, J. 2015. Mapping a large number of QTL for durable resistance to stripe rust in winter wheat Druchamp using SSR and SNP markers. PLoS One. 10(5):e0126794.
Molecular mapping of genes Yr64 and Yr65 for stripe rust resistance in hexaploid derivatives of durum wheat accessions PI 331260 and PI 480016-(Peer Reviewed Journal)
Cheng, P., Xu, L., Wang, M., See, D.R., Chen, X. 2014. Molecular mapping of genes Yr64 and Yr65 for stripe rust resistance in hexaploid derivatives of durum wheat accessions PI 331260 and PI 480016. Theoretical and Applied Genetics. 127:2267–2277.
Mapping of Yr62 and a small effect QTL for high-temperature adult-plant resistance to stripe rust in spring wheat PI 192252Reprint Icon -(Peer Reviewed Journal)
Lu, Y., Wang, M., Chen, X., See, D.R., Chao, S., Jing, J. 2014. Mapping of Yr62 and a small effect QTL for high-temperature adult-plant resistance to stripe rust in spring wheat PI 192252. Theoretical and Applied Genetics. 127:1449-1459.
High density mapping of a resistance gene to Ug99 from an Iranian landrace-(Peer Reviewed Journal)
Zurn, J.D., Newcomb, M.S., Rouse, M.N., Jin, Y., Shiaoman, C., Sthapit, J., See, D.R., Wanyera, R., Njau, P., Bonman, J.M., Brueggeman, R., Acevedo, M. 2014. High density mapping of a resistance gene to Ug99 from an Iranian landrace. Molecular Breeding. 34:871-881.
Copy number and haplotype variation at the VRN-A1 and central FR-A2 loci are associated with frost tolerance in hexaploid wheat-(Peer Reviewed Journal)
Zhu, J., Pearce, S., Burke, A., See, D.R., Skinner, D.Z., Dubcovsky, J., Garland Campbell, K.A. 2014. Copy number and haplotype variation at the VRN-A1 and central FR-A2 loci are associated with frost tolerance in hexaploid wheat. Theoretical and Applied Genetics. 127(5):1183-1197.
Genetic diversity for stripe rust Resistance in wheat landraces and identification of accessions with resistance to stem rust and stripe rustReprint Icon -(Peer Reviewed Journal)
Sthapit, J., Newcomb, M.S., Bonman, J.M., Chen, X., See, D.R. 2014. Genetic diversity for stripe rust Resistance in wheat landraces and identification of accessions with resistance to stem rust and stripe rust. Crop Science. doi: 10.2135/cropsci2013.07.0438.
Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landrace and cultivarsReprint Icon -(Peer Reviewed Journal)
Cavanagh, C., Chao, S., Wang, S., Huang, B.E., Stephan, S., Kiani, S., Forrest, K., Saintenac, C., Brown Guedira, G.L., Akhunova, A., See, D.R., Bai, G., Pumphrey, M.O., Tomar, L., Wong, D., Kong, S., Reynolds, M., Lopez Da Silva, M., Bockelman, H.E., Talbert, L., Anderson, J.A., Dreisigacker, S., Baenziger, S., Carter, A., Korzun, V., Morrell, P.L., Dubcovsky, J., Morell, M., Sorrells, M., Hayden, M., Akhunov, E. 2013. Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landrace and cultivars. Proceedings of the National Academy of Sciences. 110:8057-8062.
Comparative analysis of syntenic genes in grass genomes reveals accelerated rates of gene structure and coding sequence evolution in polyploid wheatReprint Icon -(Peer Reviewed Journal)
Akhunov, E.D., Sehgal, S., Liang, H., Wang, S., Akhunova, A.R., Kaur, G., Li, W., Forrest, K.L., See, D., Simkova, H., Ma, Y., Hayden, M.J., Luo, M., Faris, J.D., Dolezel, J., Gill, B.S. 2013. Comparative analysis of syntenic genes in grass genomes reveals accelerated rates of gene structure and coding sequence evolution in polyploid wheat. Plant Physiology. 161:252-265.
Registration of “Cara” soft white winter club wheat-(Peer Reviewed Journal)
Garland Campbell, K.A., Allan, R.E., Anderson, J., Burke, A.B., Blake, N., Hoagland, C.D., Walker, C., Chatelain, J., Little, L.M., Pritchett, J.A., Chen, X., Morris, C.F., See, D.R., Guy, S., Murray, T., Engle, D.A., Wetzel, H., Wood, D.A. 2012. Registration of “Cara” soft white winter club wheat. Journal of Plant Registrations. 7:81-88 doi:10.3198/jpr2012.01.0005crc.
Development and characterization of expressed sequence tag-derived microsatellite markers for the wheat stripe rust fungus Puccinia striiformis f. sp. tritici-(Peer Reviewed Journal)
Cheng, P., Chen, X., Xu, L., See, D.R. 2012. Development and characterization of expressed sequence tag-derived microsatellite markers for the wheat stripe rust fungus Puccinia striiformis f. sp. tritici. Molecular Ecology Resources. 12:779-781.
Mapping QTL for resistance to eyespot of wheat in Aegilops longissima-(Peer Reviewed Journal)
Identification of quantitative trait loci (QTL) for resistance to Fusarium crown rot (Fusarium pseudograminearum) in multiple assay environments in the Pacific Northwestern US -(Peer Reviewed Journal)
Poole, G., Smiley, R., Paulitz, T.C., Carter, A., See, D.R., Campbell, K. 2012. Identification of quantitative trait loci (QTL) for resistance to Fusarium crown rot (Fusarium pseudograminearum) in multiple assay environments in the Pacific Northwestern US . Theoretical and Applied Genetics. 125:91-107.
Genetic Mapping of a new family of Seed-Expressed Polyphenol Oxidase genes in Wheat (Triticum aestivum L.)Reprint Icon -(Peer Reviewed Journal)
Beecher, B.S., Carter, A.H., See, D.R. 2012. Genetic Mapping of a new family of Seed-Expressed Polyphenol Oxidase genes in Wheat (Triticum aestivum L.). Journal of Theoretical and Applied Genetics. 124:1463-1473.
Quantitative trait loci analysis for the effect of Rht-B1b dwarfing gene on coleoptile length and seedling root length and number of bread wheat-(Peer Reviewed Journal)
Li, P., Chen, J., Wu, P., Zhang, J., Chu, C., See, D.R., Brown Guedira, G.L., Zemetra, R., Souza, E.J. 2011. Quantitative trait loci analysis for the effect of Rht-B1b dwarfing gene on coleoptile length and seedling root length and number of bread wheat. Crop Science. 51:2561-2568 (2011).
Mapping and validation of Yr48 and other QTL conferring partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat-(Peer Reviewed Journal)
Lowe, I., Jankuloski, L., Chao, S., Chen, X., See, D.R., Dubcovsky, J. 2011. Mapping and validation of Yr48 and other QTL conferring partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat. Theoretical and Applied Genetics. 123:143-157.
Glutenin alleles in U.S. Pacific Northwest Wheat-(Proceedings)
Morris, C.F., Pasha, I., Beecher, B.S., Bettge, A.D., See, D.R. 2009. Glutenin alleles in U.S. Pacific Northwest Wheat. International Gluten Workshop; p 141.
Glutenin alleles in U.S. Pacific Northwest wheat-(Abstract Only)
Development of a PCR Assay and Marker-Assisted Transfer of Leaf Rust and Stripe Rust Resistance Genes Lr57 and Yr40 into Hard Red Winter WheatsReprint Icon -(Peer Reviewed Journal)
Kuraparthy, V., Sood, S., See, D.R., Gill, B.S. 2009. Development of a PCR Assay and Marker-Assisted Transfer of Leaf Rust and Stripe Rust Resistance Genes Lr57 and Yr40 into Hard Red Winter Wheats. Crop Sci. 49:120-126.
Microsatellite markers for kernel color genes in wheat-(Peer Reviewed Journal)
Sherman, J.D., Souza, E.J., See, D.R., Talbert, L.E. 2008. Microsatellite markers for kernel color genes in wheat. Crop Science. 48:1419-1424.

Projects

Genetic Improvement of Wheat and Barley for Resistance to Biotic and Abiotic Stresses
In-House Appropriated (D)
 Accession Number:424575
Discovery and Development of Wheat Germplasm with Increased Resistance to Biotic Stresses
Non-Assistance Cooperative Agreement (S)
 Accession Number:426401

 


Last Modified: 3/22/2017
Footer Content Back to Top of Page