Location: Wheat Health, Genetics, and Quality Research
Title: Genome-wide association mapping for stripe rust resistance loci in global spring barley germplasmAuthor
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UPADHAYA, ARJUN - Washington State University |
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WANG, MAINAN - Washington State University |
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FATIMA, NOSHEEN - Washington State University |
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BROOKE, MATTHEW - Washington State University |
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BRUEGGEMAN, ROBERT - Washington State University |
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Ruff, Travis |
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See, Deven |
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Chen, Xianming |
Submitted to: National American Phytopathology Meetings
Publication Type: Abstract Only Publication Acceptance Date: 6/1/2024 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Stripe rust, caused by Puccinia striiformis f. sp. hordei (Psh), is an important disease of barley worldwide. Sustainable crop protection from stripe rust can be achieved through a judicious use of genetic resistance. The objective of this study is to identify and map loci for all-stage resistance (ASR) and high-temperature adult-plant (HTAP) resistance to Psh in barley. A genome wide association study (GWAS) was conducted utilizing 320 spring barley accessions in a global core collection genotyped with the 9K barley Illumina SNP chip. The accessions were evaluated for stripe rust responses in two field environments, Mount Vernon (natural epidemic) and Pullman (artificially inoculated), WA in 2023; for ASR with four Psh races in the seedling stage at a low temperature profile and HTAP resistance with a predominant race in the adult-plant stage at a high temperature profile in the greenhouse. At the seedling stage, 8-12% accessions were resistant, 6-28% were moderately resistant, and 51-85% were susceptible. Association analysis identified 46 marker-trait associations (MTAs) (P < 8.06 × 10-6), corresponding to 26 unique loci across all seven chromosomes, including 16 for ASR and 10 for HTAP resistance. The PCR Allele Competitive Extension (PACE) markers were designed and validated for single nucleotide polymorphisms (SNPs) tagging six major loci. The findings from this study will advance our understanding of the host-pathogen interactions in the barley-Psh system and contribute to resistance breeding efforts towards sustainable disease management. |