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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #413772

Research Project: Enhancing Control of Stripe Rusts of Cereal Crops

Location: Wheat Health, Genetics, and Quality Research

Title: Genome-wide association mapping for stripe rust resistance loci in global spring barley germplasm

Author
item UPADHAYA, ARJUN - Washington State University
item WANG, MAINAN - Washington State University
item FATIMA, NOSHEEN - Washington State University
item BROOKE, MATTHEW - Washington State University
item BRUEGGEMAN, ROBERT - Washington State University
item Ruff, Travis
item See, Deven
item Chen, Xianming

Submitted to: National American Phytopathology Meetings
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2024
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Stripe rust, caused by Puccinia striiformis f. sp. hordei (Psh), is an important disease of barley worldwide. Sustainable crop protection from stripe rust can be achieved through a judicious use of genetic resistance. The objective of this study is to identify and map loci for all-stage resistance (ASR) and high-temperature adult-plant (HTAP) resistance to Psh in barley. A genome wide association study (GWAS) was conducted utilizing 320 spring barley accessions in a global core collection genotyped with the 9K barley Illumina SNP chip. The accessions were evaluated for stripe rust responses in two field environments, Mount Vernon (natural epidemic) and Pullman (artificially inoculated), WA in 2023; for ASR with four Psh races in the seedling stage at a low temperature profile and HTAP resistance with a predominant race in the adult-plant stage at a high temperature profile in the greenhouse. At the seedling stage, 8-12% accessions were resistant, 6-28% were moderately resistant, and 51-85% were susceptible. Association analysis identified 46 marker-trait associations (MTAs) (P < 8.06 × 10-6), corresponding to 26 unique loci across all seven chromosomes, including 16 for ASR and 10 for HTAP resistance. The PCR Allele Competitive Extension (PACE) markers were designed and validated for single nucleotide polymorphisms (SNPs) tagging six major loci. The findings from this study will advance our understanding of the host-pathogen interactions in the barley-Psh system and contribute to resistance breeding efforts towards sustainable disease management.