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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #338931

Research Project: Genetic Improvement of Wheat and Barley for Resistance to Biotic and Abiotic Stresses

Location: Wheat Health, Genetics, and Quality Research

Title: Mapping genes for resistance to stripe rust in spring wheat landrace PI 480035

Author
item Sthapit Kandel, Jinata - Washington State University
item Krishnan, Vandhana - Stanford University School Of Medicine
item Jiwan, Derick - Washington State University
item Chen, Xianming
item Skinner, Daniel - Dan
item See, Deven

Submitted to: PLoS One
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/1/2017
Publication Date: 5/19/2017
Citation: Sthapit Kandel, J., Krishnan, V., Jiwan, D., Chen, X., Skinner, D.Z., See, D.R. 2017. Mapping genes for resistance to stripe rust in spring wheat landrace PI 480035. PLoS One. 12(5):e0177898.

Interpretive Summary: Stripe rust is an economically important disease of wheat (Triticum aestivum L.). Hexaploid spring wheat landrace PI 480035 was highly resistant to stripe rust in the field in Washington during 2011 and 2012. The objective of this research was to identify quantitative trait loci (QTL) for stripe rust resistance in PI 480035. A spring wheat, “Avocet Susceptible” (AvS), was crossed with PI 480035 to develop a biparental population of 110 recombinant inbred lines (RIL). The population was field evaluated in 2013 and 2014 and seedling reactions were examined against three races (PSTv-14, PSTv-37, and PSTv-40) of the pathogen under controlled conditions. The population was genotyped with genotyping-by-sequencing and microsatellite markers across the whole wheat genome. A major QTL, QyrPI480035-1BS was identified on chromosome 1B. The closest flanking markers were gwm273, gwm11, and barc187 at 1.01 cM distally, cfd59 at 0.59 cM and A365 at 3.19 cM proximally. Another QTL, QyrPI480035-3B, was identified on 3B, which was significant only for PSTv-40 and was not significant in the field, indicating it confers a race-specific resistance. Comparison with markers associated with previously reported closest Yr genes on 1B (Yr64, Yr65, and YrH52) indicated that QyrPI480035-1BS is potentially a novel stripe rust resistance gene that can be incorporated into modern breeding materials, along with other all-stage and adult-plant resistance genes to develop cultivars that can provide durable resistance.

Technical Abstract: Stripe rust caused by Puccinia striiformis Westend. f. sp. tritici Erikks. is an economically important disease of wheat (Triticum aestivum L.). Hexaploid spring wheat landrace PI 480035 was highly resistant to stripe rust in the field in Washington during 2011 and 2012. The objective of this research was to identify quantitative trait loci (QTL) for stripe rust resistance in PI 480035. A spring wheat, “Avocet Susceptible” (AvS), was crossed with PI 480035 to develop a biparental population of 110 recombinant inbred lines (RIL). The population was field evaluated in 2013 and 2014 and seedling reactions were examined against three races (PSTv-14, PSTv-37, and PSTv-40) of the pathogen under controlled conditions. The population was genotyped with genotyping-by-sequencing and microsatellite markers across the whole wheat genome. A major QTL, QyrPI480035-1BS was identified on chromosome 1B. The closest flanking markers were gwm273, gwm11, and barc187 at 1.01 cM distally, cfd59 at 0.59 cM and A365 at 3.19 cM proximally. Another QTL, QyrPI480035-3B, was identified on 3B, which was significant only for PSTv-40 and was not significant in the field, indicating it confers a race-specific resistance. Comparison with markers associated with previously reported closest Yr genes on 1B (Yr64, Yr65, and YrH52) indicated that QyrPI480035-1BS is potentially a novel stripe rust resistance gene that can be incorporated into modern breeding materials, along with other all-stage and adult-plant resistance genes to develop cultivars that can provide durable resistance.