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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #325734

Research Project: Improved Control of Stripe Rust in Cereal Crops

Location: Wheat Health, Genetics, and Quality Research

Title: Molecular mapping of stripe rust resistance gene Yr76 in winter club wheat cultivar Tyee

Author
item XIANG, CHAO - Washington State University
item FENG, JUNYAN - Washington State University
item WANG, MEINAN - Washington State University
item Chen, Xianming
item See, Deven
item WAN, ANMIN - Washington State University
item WANG, TAO - Chinese Academy Of Sciences

Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/26/2016
Publication Date: 4/6/2016
Citation: Xiang, C., Feng, J., Wang, M., Chen, X., See, D.R., Wan, A., Wang, T. 2016. Molecular mapping of stripe rust resistance gene Yr76 in winter club wheat cultivar Tyee. Phytopathology. doi: 10.1094/PHYTO-01-16-0045-FI.

Interpretive Summary: Tyee is a winter club wheat and used to differentiate races of the stripe rust pathogen in the United States. The cultivar was previously identified to have a single gene for all-stage resistance, tentatively named YrTye. To map the gene, Tyee was crossed with ‘Avocet Susceptible’ (AvS). Genetic analysis of the F1, F2, F2:3, and BC1 progenies confirmed a single dominant gene for resistance to race PSTv-37 that is avirulent to YrTye. A mapping population of 135 F2 plants was phenotyped with PSTv-37 and the derived F2:3 lines were tested with races PSTv-37, PSTv-40, and PSTv-79. The mapping population was genotyped with simple sequence repeat (SSR) markers. A genetic map comprising thirteen SSR markers located YrTye in chromosome 3AS flanked distally by SSR marker Xwmc11 and proximally by Xwmc532 at 2.6 and 3.4 cM, respectively. Amplification of Chinese Spring 3A deletion lines placed the gene in the distal part of the chromosome 3AS. Based on unique chromosomal location, we permanently name the gene Yr76. A near-isogenic line of spring common wheat was developed. The specific SSR alleles flanking Yr76 were validated their high polymorphisms were determined using various wheat cultivars. The specificity of Yr76 is useful in differentiating races of the stripe rust pathogen, and its tightly linked markers will be useful in developing resistant cultivars when combining Yr76 with other genes for resistance to stripe rust.

Technical Abstract: Tyee, one of the wheat cultivars used to differentiate races of Puccinia striiformis f. sp. tritici (Pst) in the United States, was identified to have a single gene for all-stage resistance, tentatively named YrTye. To map the gene, Tyee was crossed with ‘Avocet Susceptible’ (AvS). Genetic analysis of the F1, F2, F2:3, and BC1 progenies confirmed a single dominant gene for resistance to race PSTv-37 that is avirulent to YrTye. A mapping population of 135 F2 plants was phenotyped with PSTv-37 and the derived F2:3 lines were tested with races PSTv-37, PSTv-40, and PSTv-79. The F2 mapping population was genotyped with simple sequence repeat (SSR) markers. A genetic map comprising thirteen SSR markers located YrTye in chromosome 3AS flanked distally by SSR marker Xwmc11 and proximally by Xwmc532 at 2.6 and 3.4 cM, respectively. Amplification of Chinese Spring 3A deletion lines placed the gene in the distal bin 3AS4-0.45–1.00. As YrTye is different from all formally named Yr genes in chromosomal location, we permanently name the gene Yr76. A near-isogenic line of spring common wheat was developed and selected by testing F3 lines derived from a AvS*4/Tyee cross with Tyee-avirulent and virulent races and the flanking markers. The specific SSR alleles flanking Yr76 were validated using cultivars and breeding lines with and without the gene, and showed highly polymorphisms in other genotypes. The specificity of Yr76 is useful in differentiating Pst races, and its tightly linked markers will be useful in developing resistant cultivars when combining Yr76 with other genes for resistance to stripe rust.