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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #311339

Research Project: Improved Control of Stripe Rust in Cereal Crops

Location: Wheat Health, Genetics, and Quality Research

Title: Mapping a large number of QTL for durable resistance to stripe rust in winter wheat Druchamp using SSR and SNP markers

Author
item HOU, LU - Washington State University
item Chen, Xianming
item WANG, MEINAN - Washington State University
item See, Deven
item Chao, Shiaoman
item BULLI, PETER - Washington State University
item JING, JINXUE - Northwest Agriculture And Forestry University

Submitted to: PLoS ONE
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/7/2015
Publication Date: 5/13/2015
Citation: Hou, L., Chen, X., Wang, M., See, D.R., Chao, S., Bulli, P., Jing, J. 2015. Mapping a large number of QTL for durable resistance to stripe rust in winter wheat Druchamp using SSR and SNP markers. PLoS One. 10(5):e0126794.

Interpretive Summary: Winter wheat Druchamp has both high-temperature adult-plant (HTAP) resistance and all-stage resistance to stripe rust. The HTAP resistance in Druchamp is durable as the variety has been resistant in adult-plant stage since it was introduced from France to the United States in late 1940s. To map the quantitative trait loci (QTL) for stripe rust resistance, a recombinant inbred line population from cross Druchamp × Michigan Amber was phenotyped for stripe rust response in multiple years in fields under natural infection and with selected races of the stripe rust pathogen under controlled greenhouse conditions; and genotyped with simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. Eight QTL for HTAP resistance and three QTL for all-stage resistance were identifed and mapped to chromosomal regions. Four of the HTAP resistance QTL were significant in all tests; and the other four QTL were significant in some of the tests. The three all-stage resistance QTL were detected based on reactions in seedlings tested with certain races. The all-stage resistance QTL on chromosome 5DL is a new resistance gene. All these QTL, especially those for durable HTAP resistance, and their closely linked molecular markers could be useful for developing wheat cultivars with durable resistance to stripe rust.

Technical Abstract: Winter wheat Druchamp has both high-temperature adult-plant (HTAP) resistance and all-stage resistance to stripe rust caused by Puccinia striiformis f. sp. tritici (Pst). The HTAP resistance in Druchamp is durable as the variety has been resistant in adult-plant stage since it was introduced from France to the United States in late 1940s. To map the quantitative trait loci (QTL) for stripe rust resistance, an F8 recombinant inbred line (RIL) population from cross Druchamp × Michigan Amber was phenotyped for stripe rust response in multiple years in fields under natural infection and with selected Pst races under controlled greenhouse conditions; and genotyped with simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. Composite interval mapping (CIM) identified eight HTAP resistance QTL and three all-stage resistance QTL. Among the eight HTAP resistance QTL, QYrdr.wgp-1BL.2 (explaining 2.36-31.04 % variation), QYrdr.wgp-2BL (2.81-15.65 %), QYrdr.wgp-5AL (2.27-17.22 %) and QYrdr.wgp-5BL.2 (2.42–15.13 %) were significant in all tests; and QYrdr.wgp-1BL.1 (1.94-10.19 %), QYrdr.wgp-1DS (2.04-27.24 %), QYrdr.wgp-3AL (1.78-13.85 %) and QYrdr.wgp-6BL.2 (1.69-33.71 %) were significant in some of the tests. The three all-stage resistance QTL, QYrdr.wgp-5BL.1 (5.47-36.04 %), QYrdr.wgp-5DL (9.27-11.94 %) and QYrdr.wgp-6BL.1 (13.07-20.36 %), were detected based on reactions in seedlings tested with certain Pst races. All-stage resistance QTL QYrdr.wgp-5DL with the nearest SNP marker IWA8331 appeared to be a new resistance locus. All these QTL, especially those for durable HTAP resistance, and their closely linked molecular markers could be useful for developing wheat cultivars with durable resistance to stripe rust.