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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Research Project #441894

Research Project: Leveraging High-Throughput Genotyping and Phenotyping Technologies to Accelerate Wheat Improvement

Location: Wheat Health, Genetics, and Quality Research

Project Number: 2090-21000-033-031-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Oct 1, 2022
End Date: Dec 31, 2022

Objective:
1) Develop cost-effective medium-density Single Nucleotide Polymorphism (SNP) assays incorporating both functional and haplotype-tagging SNPs for effective genome-wide imputation and link these assays with Practical Haplotype Graph (PHG) to establish a centralized automated platform for GS in public wheat breeding programs. 2) Develop improved tools for T3/BreedBase to accelerate the automatic deposit of genotypic and agronomic data from public breeding programs into Triticeae (T3), and actively promote the exchange of data and analyses tools with equivalent public wheat databases in International Maize and Wheat Improvement Center (CIMMYT) and the United Kindom (UK).

Approach:
“Genotyping by Multiplex Sequencing” (GMS) approach, that targets thousands of single nucleotide polymorphisms (SNP) in 7 PCR pools, and yields ~2000 genotypes per wheat line.