|Heaton, Michael - Mike|
|Smith, Timothy - Tim|
|Kappes, Steven - Steve|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/1998
Publication Date: N/A
Technical Abstract: The development of a bovine genetic map has provided new opportunities for improving herd health and food safety in beef cattle. Our aim was to identify and map bovine genes that are expressed in response to pathogen infection with the ultimate goal of evaluating allelic variants for association with infection phenotypes. RNA fingerprinting by arbitrary primers (RAP)-PCR was used to identify two bovine genes that were expresse in response to treatment of epithelial cells (MDBK) with a combination of E. coli O157:H7 lipopolysaccharide (LPS) and cycloheximide. The two bovine cDNA sequences were homologous to those encoding human C-X-C chemokines interleukin 8 and macrophage inflammatory protein-2 beta, both of which possess potent chemotactic activity for neutrophils. An increase in the two transcript levels in MDBK cells was confirmed by Rnase protection assays. SNPs were identified for each locus and used to determine their location on the bovine genetic map. Linkage analysis indicated that both genes lie in a region of bovine chromosome 6 that corresponds to human chromosome 4 (q12-21) where the chemokine alpha gene cluster resides. These results illustrate on approach for identifying and mapping candidate genes for infection-resistance in cattle.