Submitted to: Annual Meeting and Expo of the American Oil Chemists' Society
Publication Type: Abstract Only
Publication Acceptance Date: 5/10/1998
Publication Date: N/A
Technical Abstract: Reversed-phase high performance liquid chromatography (RP-HPLC) coupled with atmospheric pressure chemical ionization mass spectrometry (APCI-MS) was used for analysis of human chylomicron triacylglycerols (TAGs) collected from subjects that were fed normal or randomized lard. Samples were collected at 0 hr, 4 hr and 6 hr after feeding, and were analyzed in triplicate. Quantitative analysis was performed using response factors calculated by comparison of the fatty acid (FA) composition calculated from the TAG composition determined using LC/APCI MS to the FA composition determined by calibrated GC FID. The FA compositions calculated from the TAG compositions changed over time in the direction of the FA composition of the lard fed. The amount of stearic acid, which showed the greatest difference between lard and 0 hr samples, increased more substantially than any other FA in both normal and randomized lard fed subjects. The change in the amount of stearic acid over time was larger for normal lard fed subjects than for randomized lard fed subjects. Similarly, the amount of linoleic acid changed more for subjects fed normal lard than those fed randomized lard. Several specific molecular species that were present in larger amounts in the lard than in plasma showed particularly large relative changes in the chylomicron TAG composition over time compared to 0 hr samples. These included POS, OOS, SSO, SSP, PPS, PPP, and PLS. Molecular species that were present in smaller amounts in the lard than in plasma decreased over time. These included OOP, PLO, and LLP.