Submitted to: American Society for Virology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 7/11/1998
Publication Date: N/A
Technical Abstract: The braconid wasp Glyptapanteles indiensis infects its natural host, Lymantria dispar, with a polydnavirus (GiPDV) to suppress the immune system during parasitization. Part of the GiPDV viral genome persisted in a GiPDV-infected L. dispar cell line (LdEp) of somatic origin. DNA from at least two of approximately 11 ds circular DNA segments comprising the GiPDV genome was maintained in vitro. GiPDV DNA was integrated, as physical linkage of GiPDV and LdEp cellular DNA was demonstrated by cloning and sequencing of an integration junction. The precise integration site was identified by comparing restriction and sequences of GiPDV circular and integrated forms. GiPDV sequences in the vicinity of the site of integration were analyzed and putative repeats identified. Southern hybridization and PCR assays also indicated that GiPDV DNA was integrated and not maintained as an episome. Several insect cell lines were evaluated for the ability to be transformed by GiPDV DNA in vitro.