Submitted to: Coccidiosis International Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/30/1998
Publication Date: N/A
Technical Abstract: The species-specificity and cross-reactivity of chicken monoclonal antibodies (mAbs) developed against E. acervulina (EA) sporozoite were determined by confocal laser scanning microscopy and Western blotting analysis using the sporozoites of seven different avian Eimeria species such as EA, E. brunetti (EB), E. maxima (EM), E. mitis, E. necatrix, E. praecox and E. tenella. The five different mAbs, named 8E-1, HE-4, 8D-2, 5D-11 and 8C-3 were used in this study. In the immunofluorescent examination, these mAbs showed similar reactivity on the apical complex of the sporozoite. One of these mAbs, 8E-1, cross-reacted with all Eimeria species that were examined, HE-4 and 8D-2 mAbs reacted with EA and EB, 5D-11 mAb reacted with EA and EM, 8C-3 mAb reacted with only EA. In Western blot analysis using sonicated sporozoites of the seven of the Eimeria species as antigen, all mAbs recognized multiple bands; the predominant bands of mAbs had molecular weights of approximately 32, 43 and 260 kilodaltons. Collectively, the results indicate that various antigenic epitopes express on the sporozoite apical complex of the Eimeria species, that is, common antigenic epitopes as recognized with 8E-1, interspecies-specific epitopes as recognized with HE-4, 8D-2 and 5DS-11, and species-specific epitopes as recognized with 8C-3.