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Title: INFLUENCE OF CIS-9, TRANS-12- AND TRANS-9, CIS-12-OCTADECADIENOIC ACID ON CHOLESTEROL METABOLISM IN HUMAN HEPG2 CELLS

Author
item YANAGITA, T - SAGA UNIVERSITY JAPAN
item YOTSUMOTO, H - SAGA UNIVERSITY JAPAN
item YAMAMOTO, K - SAGA UNIVERSITY JAPAN
item SUGANO, M - KYUSHU UNIVERSITY JAPAN
item Emken, Edward

Submitted to: Annual Meeting and Expo of the American Oil Chemists' Society
Publication Type: Abstract Only
Publication Acceptance Date: 5/12/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: This study examined the effect of trans 18:2 fatty acid isomers on the cholesterol synthesis and metabolism in the human hepatoma cell line HepG2. Linoleic acid (CC) supplemented and non-supplemented culture media was used to determine if the presence of linoleic acid influenced the effect of c,t-18:2 and t,c-18:2 on cholesterol synthesis. In experiment 1, cells were cultured in the linoleic acid-free medium supplemented with 9c, 12t-18:2-d4 (CT), 9t,12c-18:2-d2 (TC), 9t, 12t-18:2 (TT) or 9c, 12c-18:2 (CC) absorbed on 0.5% BSA. In experiment 2, cells were cultured in a linoleic-enriched medium supplemented with 0.1 mM CT, TC, TT or CC. At 24 hrs incubation, TC, TT and the highest concentration of CT increased the incorporation of [14C]acetate into both free and esterified cholesterol. In contrast, for cells incubated in the linoleic-enriched medium, there was no significant differences in the incorporation of [14C]acetate into cellular cholesterol fractions after 24 hrs. In linoleic acid-free medium, TC and the highest concentration of CT increased the incorporation of [3H]glycerol into triglyceride, whereas TT decreased incorporation. In conclusion, cholesterol synthesis in HepG2 cells was stimulated by the trans 18:2 fatty acid isomers in a linoleic acid-free medium, but not in linoleic acid-enriched medium. These results suggested that the presence of linoleic in the culture medium inhibited the effect of trans 18:2 isomers on cholesterol synthesis in HepG2 cells.