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Title: PREVENTING THE INSULIN-INDUCED FALL IN AMINO ACIDS VIA VARIABLE INFUSION OFAN AMINO ACID MIXTURE INCREASES THE GLUCOSE RESPONSE TO INSULIN SUCKLING PIGS.

Author
item Wray Cahen, Diane
item BECKETT, PHILIP
item NGUYEN, HANH
item REEDS, PETER
item ALLNUTT, DEBBIE
item DAVIS, TERESA

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/5/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The neonate has a high growth rate and the fractional rate of growth declines rapidly with age. Insulin (INS) is a key factor in the regulation and coordination of the post-absorptive use of nutrients. We performed INS dose response studies in 7- and 26-d-old suckling pigs, using a hyper- insulinemic-euglycemic clamp technique with (n=10) or without (n=8) clamping AA. We determined: 1) the effect of maintaing near basal levels of AA under hyperinsulinemic conditions on whole-body GLC disposal, 2) the effect of INS on whole-body AA disposal, and 3) the effect of stage of development on these parameters. GLC alone or GLC and AA were maintained at basal fasting levels by variable infusions of dextrose alone or dextrose and an AA mixture. GLC and AA disposal were estimated from the infusion rates of dextrose (DIR) and AA (AAIR), respectively. In 7-d-old pigs, clamping AA near basal levels increased the maximum GLC disposal response to INS (47.6+3.3 vs. 54.7+2.5 mg glucose/kg/min; -AA vs. +AA). The INS- dose response curve for DIR was shifted to the left at both ages and there- fore, clamping AA increased the sensitivity of GLC disposal to INS> In 7- compared to 26-d-old pigs, the maximum response for AAIR was 2-fold higher and the INS-dose response curve was shifted to the left. The results indicate that maintaining AA supply increases the DIR response to INS in young pigs. The data from the present study, in combination with the data from adults, suggest that the responses of GLC and AA metabolism to INS are dependent on stage of development and decline with age. The heightened sensitivity and responsiveness to INS for GLC disposal in the nenonate may play an important role in supporting the energetic needs for its high rates