Author
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ELIAS, KAROL - NORTH CAROLINA STATE |
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Submitted to: Fungal Genetics and Biology
Publication Type: Abstract Only Publication Acceptance Date: 4/15/1997 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Komada's medium (Komada 1975 Rev. Plant Protec. Res. 8:114-125) was developed as an agar-based selective medium to isolate Fusarium oxysporum from raw soil or infested plant material. Typically, a soil dilution extract or plant material is placed on the agar surface or just slightly pushed into the agar. In my hands, other fungi and bacteria can grow under rthese conditions presumably by utilizing the soil nutrients or decaying plant material. Thus it is not always possible to obtain pure cultures of F. oxysporum from a Komada's agar plate surface. I have found that if you overlay the soil extract or plant material with a second layer of Komada's medium by "flipping" the contents of an additional Komada's medium agar plate onto the first, it is then possible to obtain a pure culture of F. oxysporum. Let the plate incubate at room temperature (25-28 C) for 7-14 days or until mycelium appears on the surface of the overlay and then transfer the fuzzy aerial mycelial growth to a new plate of any general fungal growth media (ie. Czapeks or PDA). It appears that by forcing the fungal culture to grow through the thickness of the overlay, the ability of Komada's medium to eliminate or selectively exclude other bacteria and fungi is dramatically enhanced. |
