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United States Department of Agriculture

Agricultural Research Service


item De Gortari, Mauricio
item Freking, Brad
item Kappes, Steven - Steve
item Leymaster, Kreg
item Crawford, Allan
item Stone, Roger
item Beattie, Craig

Submitted to: Animal Genetics
Publication Type: Abstract Only
Publication Acceptance Date: 4/17/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The significant homology of chromosomal banding structures between cattle, sheep and goats suggests a homologous genetic structure and karyotypic conservation that simplifies synteny mapping. Genome conservation extends to microsatellite (ms) loci among bovid and caprid species. These tandemly repeated, highly polymorphic, single locus DNA sequences are well distributed over numerous mammalian genera, and are readily adaptable to PCR systems. As such, they are the marker of choice for establishing linkage, estimating genetic diversity, and studying evolutionary relationships. Primer pairs developed for ms loci derived from cattle and sheep have recently been reciprocally assigned to linkage groups on homologous chromosomes within each species. However, the extent to which a comparative linkage strategy can be used to provide a robust estimate of informativeness and genome coverage, calculate (genetic) breakpoints about syntenic regions, and improve the resolution around an ETL(s) is currently hampered by the limited number of ms informative across species. We report the evaluation of 1,065 primer pairs for bovine ms for their suitability as (linkage) markers in sheep. This includes 82 bovine primer pairs previously screened across several sheep populations. All sequences have been deposited in either the GenBank or USDA-ARS-MARC databases.

Last Modified: 06/26/2017
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