Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/12/1996
Publication Date: N/A
Technical Abstract: Thermophilic Campylobacter, Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli), can survive for extended periods in fecal specimens and are found in surface water. The species C. coli is a much less common cause of human disease. Campylobacter coli is closely related to C. jejuni, and their infections appear to share many clinical and epidemiologic characteristics. Human infection with C. jejuni/coli can result in a range of clinical illness from transient asymptomatic colonization to severe dysentery. The usual manifestation of infection is acute enteritis. We examined 605 samples of feces from a total of 26 farms during the months of May and June. A multiplex polymerase chain reaction (mPCR) using primers pg3, pg50, C-1, and C-4 were used to discriminate 605 fecal specimens for C. jejuni and C. coli. By PCR, of 605 samples analyzed, 158 (26.11%) were confirmed positive for C. jejuni and 16 (2.64%) positive for C. coli. The recovery of C. jejuni ranged from 0 to 48%. This study shows the prevalence of thermophilic Campylobacter in dairy market cattle in the United States.