|Webber, Charles - Chuck|
Submitted to: Fifth Chemical Congress of North America
Publication Type: Abstract only
Publication Acceptance Date: 11/11/1997
Publication Date: N/A
Citation: Interpretive Summary:
Technical Abstract: A practical genetic transformation method for kenaf requires that the system is rapid genotype independent, and results in high frequency of transformants. A system is described which uses an easily regenerated explant, the 3-to-4-day-old shoot apex, and Agrobacterium tumefaciens as the gene vector. Shoot apices were isolated, surface sterilized, and germinated on a Murashige and Skoog (MS) salts medium. Shoot apices were co-cultivated with different Agrobacterium tumefaciens strains, LBA4404, EHA101S and Z707S, containing genes for resistance to phosphinotricin (ppt) and hygromycin (hyg). After co-cultivation for 3 days in the light, shoot apices were transferred to the selective medium containing 10 mg/l ppt or 16 mg/l hyg formed roots on MS medium. The highest percentages of rooted plants on selective medium were obtained from shoots that were co-cultivated with Agrobacterium strain LBA4404.