Submitted to: International Marek's Disease Symposium Abstracts and Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 9/7/1996
Publication Date: N/A
Citation: Interpretive Summary:
Technical Abstract: The recent development of a genetic map of the chicken enabled us to identify quantitative trait loci (QTL) affecting susceptibility to Marek's disease (MD). The highly inbred Lines 63 (MD resistant) and 72 (MD susceptible) were mated to create 300+ F2 chickens. The F2 chickens were challenged with MD virus (MDV) at 1 week of age. The chickens were monitored for symptoms of MD susceptibility up to 10 weeks of age and all were necropsied to evaluate gross MD tumor and lesions; MDV viremia levels were determined 2 weeks post-challenge. Parametric and non-parametric ANOVA techniques were used in the QTL analysis. At the first stage of analysis, 65 DNA markers selected from the East Lansing genetic map were genotyped in 80 selected F2 chickens, and 22 markers residing near to potential QTLs affecting MD susceptibility were identified (P is less than or equal to 0.2). At the second stage of analysis, significant markers at the first stage plus flanking markers (42 in total) were genotyped on the entire F2 population. Eight QTLs (P is less than or equal to 0.01), each affecting several components of MD susceptibility, were identified on seven linkage groups of the chicken.