Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/15/1996
Publication Date: N/A
Technical Abstract: P. haemolytica A1 was incubated in bovine subcutaneous tissue chambers and heated ovine or bovine serum (also treated with protein G to remove gamma globulin) to assess changes in morphology and lectin agglutination profiles. Cells, removed from chambers after 2 h, were covered with an extensive, dense glycocalyx extending 0.5 um from the cell. Cells, removed at 6 h, had a sparse glycocalyx of fine fibers 0.2 to 0.3 um from the cell. P. haemolytica incubated for 2 h in ovine or bovine serum were also covered with a sparse glycocalyx. The glycocalyx did not bind Protein A colloidal gold and therefore did not contain aggregates of accumulated antibody. This surface, induced after 2 h incubation in ruminant serum, altered lectin agglutination profiles indicating that some surface carbohydrate moieties were masked by serum components possibly absorbed to the cell.