Submitted to: Molecular Endocrinology
Publication Type: Other
Publication Acceptance Date: 7/23/1996
Publication Date: N/A
Technical Abstract: This paper is a review of the book entitled "ELISA: Theory and Practice. Methods in Molecular Biology, Vol.42," by John R. Crowther (Humana Press, Totowa, New Jersey). The book is intended to be an introduction to Enzyme Linked Immunosorbent Assay (ELISA) technology. The book is written for persons in the biological and clinical sciences, with and without experience using the technique. The book is divided into two main sections. The first section is comprised of an introduction to the basic underlying biological principles of the immune response as well as a description of the various ELISA formats. The second section is a compilation of several experimental protocols representative of each ELISA format and includes useful segments on both data analysis and interpretation. The book will be an effective guide for short courses or workshops on ELISA techniques, especially as it applies to clinical medicine.