Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/1/1995
Publication Date: N/A
Technical Abstract: Integration of genetic and cytogenetic maps into a comprehensive linkage map will verify sequential order, estimate chromosomal coverage by linkage groups, and detect differential recombination rates within the genome. Our effort to construct a porcine physical map involves two approaches: fluorescence in situ hybridization (FISH) and direct in situ single-copy PCR (DISC-PCR). We have recently used FISH to physically assign 30 informative markers most of which are on current porcine linkage maps. However, two of the cosmids, MS Sw2415 and MS Sw2419, were in a small, unassigned linkage group. Physically anchoring them to the distal end of chromosome 6q assigned the entire linkage group to this chromosomal position. A novel technique developed in lab. termed DISC-PCR, combines on-slide PCR with in situ localization of unique sequences. It allows direct mapping of extremely short, single-copy sequences, such as MS or expressed sequence tags, without the necessity of having large genomic clones. Using this procedure, two important POU domain genes. PIT1 and OCT2, were physically assigned to chromosomes 13 and 6, respectively, which correspond to previous linkage information. Thus, the use of FISH (when large genomic clones are available) and DISC-PCR (when only short sequences and/or primer pairs are available) will contribute to the development of a comprehensive porcine linkage map.