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ARS Home » Research » Publications at this Location » Publication #71622


item Ziprin, Richard

Submitted to: Avian Diseases
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/13/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary: Salmonella bacteria are well known as a cause of food poisoning in humans. One of the ways we can get this bacteria in our bodies is the consumption of contaminated chicken or eggs. We have conducted studies to find ways to reduce or eliminate these bacteria from poultry and poultry products. In the present study, different Salmonella strains were examined to determine how certain characteristics of the bacteria affect the chicken's resistance to infection. This work is part of a broad effort to understand the interaction between Salmonella bacteria and poultry, and is of interest to scientists attempting to find better ways to protect the public from Salmonella food poisoning.

Technical Abstract: Previous studies at this laboratory have shown that Salmonella enteritidis-immune lymphokine (ILK) promotes the migration of heterophils to the chicken peritoneum in response to Salmonella enteritidis (SE) challenge. Others have shown with mammalian experiments that migration of neutrophils is partly induced by interleukins produced when intestinal epithelial cells interact with salmonellae. The present work compared the accumulation of intraperitoneal heterophils in day-of-hatch chicks following treatment with ILK and challenge with various strains of SE. All work was done in a biosafety level 3 facility. One strain was a wild type, another was an invasion capable avirulent vaccine strain (designed for oral vaccination), and a third contained mutations that reduced invasiveness. Day-of-hatch chicks received ILK by intraperitoneal injection and were challenged 1 hr later by intraperitoneal inoculation with one of the following SE strains: a wild type, SE 890034-3; a cya-12 and crp-11 deletion avirulent vaccine strain, chi-4357; and an invasionless strain, InA::kan, chi-4420. Four hours after challenge heterophils were recovered from the peritoneal cavity by lavage. The concentration of heterophils in the recovered lavage fluid was determined. Heterophil concentrations increased in response to challenge with each SE strain but there was a lower response to the invasionless strain. The difference was statistically significant. This diminished heterophil response to challenge with invasionless salmonellae supports existing evidence that the initial defensive reaction occurs at the earliest stages of Salmonella-host interaction.