Submitted to: Lipids
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/29/1996
Publication Date: N/A
Interpretive Summary: Fats constitute a significant proportion of the average diet. The type of fat consumed is important and can be correlated with certain markers of coronary heart disease. Therefore, it is important to be able to determine the composition of a fat with as much accuracy as possible in order to assess its likely nutritional and health value. We are reporting here the use of a new analytical method to determine the amounts and types of fat components from a natural source, corn oil. We developed a new method for quantitation in an easy and straightforward way which is applicable to samples of any type. The method developed for corn oil provides us with an analytical approach to use for the analysis of human plasma samples.
Technical Abstract: Atmospheric pressure chemical ionization mass spectrometry was used for qualitative and quantitative analysis of triglycerides (TGs) in normal and randomized corn oil samples separated by reversed-phase high performance liquid chromatography. Quantitation of normal and randomized corn oils is presented which is based on response factors obtained from homogeneous TG standards, based on response factors obtained from a synthetic mixture of heterogeneous TGs, based on response factors obtained from the triglyceride quotient, based on response factors obtained from randomized corn oil, and based on response factors obtained from gas chromatographic analyses. The least relative error is obtained when quantitation is based on a mixture of TGs which is similar in composition and concentration to the analyte.