Submitted to: Journal of Animal Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/1/1995
Publication Date: N/A
Interpretive Summary: We have developed a strategy that merges the genetic and physical linkage map of closely related livestock species into relatively high-resolution comprehensive maps. The strategy provides a basis for the continued development and integration of the linkage maps essential to a systematic whole genome approach to dissection of economically important traits in livestock.
Technical Abstract: Remarkable progress has recently been made in the development of low resolution genetic linkage maps for the major livestock species. However, the several linkage maps now available for domestic species are not integrated and estimates of overall coverage (genome size) are not robust. Continued development of high resolution linkage maps will require constructing integrated framework as well as comprehensive maps, which in turn, requires increasing marker density in areas of low resolution. A strategy of directed (linkage) mapping that incorporates microdissection and microcloning of specific chromosomal regions, direct selection of large genomic DNA inserts containing polymorphic markers, and direct chromosomal amplification of previously linked loci will increase marker density in specific regions. This strategy will also aid integration of the genetic and physical maps of individual species by providing framework markers. When this strategy is extended into closely related species, e.g., cattle and sheep, well ordered comparative linkage maps can be developed that allow marker integration across species lines.