Submitted to: American Society for Microbiology
Publication Type: Abstract Only
Publication Acceptance Date: 5/23/1996
Publication Date: N/A
Technical Abstract: The genus Arcobacter encompasses the aerotolerant campylobacter-like organisms. The purpose of this study was to determine the distribution of plasmids in field isolates of Arcobacter (n=80) recovered from livestock and humans. Preliminary studies indicated that the rapid boiling method was more effective in plasmid isolation than the alkaline lysis. Plasmids were detected in 6% (1 of 17) of the A. skirrowii, in 36% (18 of 50) of the A. butzleri, and in 54% (7 of 13) of the A. cryaerophilus strains. Plasmids ranged in size from >16.2 to 3.1 kb. Eight plasmid profiles were detected. A single profile, consisting of two plasmids (6.3 and 14 kb), was found in A. cryaerophilus. All 8 profiles were present in A. butzleri. Overall, plasmids were detected in 33% (26/80) of field strains examined, which was comparable to the reported distribution of plasmids in Helicobacter pylori (39%) but lower than that of Campylobacter jejuni (55% to 100%). This represents the first report of plasmids in Arcobacter.