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ARS Home » Research » Publications at this Location » Publication #65217
Title: DETECTION OF ARCOBACTER SPECIES IN GASTRIC SAMPLES FROM SWINE

Author
item SUAREZ, DAVID - 3630-14-00
item Wesley, Irene
item LARSON, D - IOWA STATE UNIVERSITY

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/14/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Swine stomach samples were surveyed for evidence of Arcobacter sp. and Helicobacter sp. infections associated with gastric ulceration. A nested PCR test targeted to the 16S rRNA was developed that could detect many Arcobacter sp. and Helicobacter sp., and an internal oligo probe was used for differentiation and confirmation of the PCR product. The stomach tissue samples were divided into two groups, the nonglandular and glandula samples, and 86 swine stomachs were examined. 52.3% of swine stomachs tested had evidence of infection from these groups, and 77.7% of the positive samples were identified as A. butzleri using a probe that under high stringency conditions is specific for A. butzleri. Samples tested by PCR were more likely to be positive from the nonglandular samples, 44.2%, than from the glandular samples, 24.4%. Gross lesions of any stage of gastric ulceration, ranging from parakeratosis, erosions, and ulcerations, were observed in 24.4% of stomachs examined. Samples positive by PCR with lesions were 12.8%, as compared to samples negative by PCR with lesions which were 11.6%. The majority of PCR positive samples, 38.6% had no gross lesions. A single step PCR that was more specific to Arcobacter sp. was used on the nonglandular stomach samples and 10.4% of the 86 samples were positive. Arcobacter sp. were cultured from 4 of the sample stomachs, and sequencing of part of the 16S rRNA gene identified 2 of the isolates of being A. butzleri, 1 isolate as being A. cryaerophilus, and the fourth isolate being a mixed culture of A. butzleri and another Arcobacter sp. A single step PCR targeted to the urease gene and culturing methods were used to specifically look for H. pylori or other closely related urease (+) bacteria, but none were found.