|GALLAGHER JR., D|
|Kappes, Steven - Steve|
Submitted to: Colloquium on Domestic Animal Cytogenetics and Gene Mapping
Publication Type: Proceedings
Publication Acceptance Date: 7/17/1995
Publication Date: N/A
Technical Abstract: The cattle gene map has grown exponentially in the past two years with the publication of two major linkage maps based in part upon earlier information from the rodent-cattle somatic cell hybrid panel of Womack. In situ hybridization of type 1 markers was used to anchor many of the linkage groups and to tie the cattle map to human homologous chromosomes. To this end, we have mapped LDHA and IGF2 to cattle chromosome 29, using the t(1;29) chromosome and in situ hybridization. A 1.7 kb EcoRI fragment of the human cDNA of lactate dehydrogenase-A (LDHA) was tritium labeled yielding 349 grains, 15 (4.3%) on the 29 (Zmax=3.77, P<0.01). A 1.0 kb EcoRI fragment of the human insulin-like growth factor-2 (IGF2) was tritium labeled yielding 440 grains, 25 (5.7%) on the 29 (Zmax=6.5, P<0.01). LDHA was mapped to human 11p14.3 and IGF2 to human 11p15.4. Both were previously assigned to cattle U7. Although both LDHA and IGF2 appear to map near the terminal end of cattle chromosome 29, it is impossible to order them relative to one another due to the error associated with localization of tritium grains. Banding was not done because it would not have helped resolve this. Foreman et al. (1994) have recently shown that tyrosinase, which results in tyrosinase-negative albinism, is syntenic with LDHA in cattle. Therefore tyrosinase should also map to cattle chromosome 29.